Supplementing alpha-tocopherol (vitamin E) and vitamin D3 in high fat diet decrease IL-6 production in murine epididymal adipose tissue and 3T3-L1 adipocytes following LPS stimulation
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2011
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Background: It is well known that high fat diets (HFDs) induce obesity and an increase in proinflammatory
adipokines. Interleukin-6 (IL-6) is considered the major inflammatory mediator in obesity. Obesity is associated with
a vitamin deficiency, especially of vitamins E and D3. We examined the effects of vitamin D3 and vitamin E
supplementation on levels of IL-6 and IL-10 (as a marker of anti-inflammatory cytokines since, a balance between
pro- and anti-inflammatory cytokines is maintained) protein expression in adipose tissue of mice provided with an
HFD. Additionally, we measured the effects of vitamin E and vitamin D3 treatment on LPS-stimulated 3T3-L1
adipocytes IL-6 and IL-10 secretion.
Results: IL-6 protein levels and the IL-6/IL-10 ratio were decreased in epididymal white adipose tissue in groups
receiving vitamins E and D3 supplementation compared to the HFD group. A 24-hour treatment of vitamin D3 and
vitamin E significantly reduced the IL-6 levels in the adipocytes culture medium without affecting IL-10 levels.
Conclusions: Vitamin D3 and vitamin E supplementation in an HFD had an anti-inflammatory effect by decreasing
IL-6 production in epididymal adipose tissue in mice and in 3T3-L1 adipocytes stimulated with LPS. Our results
suggest that vitamin E and D3 supplementation can be used as an adjunctive therapy to reduce the
proinflammatory cytokines present in obese patients.
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LIRA, Fábio S. et al. Supplementing alpha-tocopherol (vitamin E) and vitamin D3 in high fat diet decrease IL-6 production in murine epididymal adipose tissue and 3T3-L1 adipocytes following LPS stimulation. Lipids in Health and Disease, London, v. 10, n. 37, p. 1-5, 2011.