Comparison of Leifsonia xyli subsp. xyli molecular detection in heat-treated sugarcane setts
Carregando...
Data
2019
Título da Revista
ISSN da Revista
Título de Volume
Editor
João Batista Duarte
Resumo
The thermotherapy of sugarcane setts is currently the
primary management method for Leifsonia xyli subsp. xyli (Lxx),
in Brazil. When they are immersed, the enzymes and proteins of
the bacterial cell are denatured without harming the setts buds.
Due to possible escapes from detection and consequent bacterium
survival to thermotherapy, what may result in asymptomatic
seedlings, this study aimed to detect the Leifsonia xyli subsp.
xyli bacterium in sugarcane setts using molecular techniques and
different time and temperature combinations, with or without the
addition of antibiotics. The conventional PCR method detected
the Lxx bacterial DNA only in the positive control, consisting
of a highly susceptible plant with a high bacterial concentration.
Using the nested-PCR, the Lxx DNA was detected in all the
treatments used. Thus, none of the treatments adopted in the
thermotherapy was able to eliminate the Lxx from the setts, and
the use of kasugamicin also did not eliminate the bacterium,
but reduced the bacterial population in the tested treatments.
These results confirm that the nested-PCR is a useful tool to
detect the presence of this phytobacterium in setts that will be
used as seedlings.
Descrição
Palavras-chave
Heat treatment, Ratoon stunting disease, Polimerase chain reaction, Termoterapia, Raquitismo-das-soqueiras, Reação em cadeia da polimerase
Citação
DIAS, Vanessa Duarte; CARRER FILHO, Renato; CUNHA, Marcos Gomes da. Comparison of Leifsonia xyli subsp. xyli molecular detection in heat-treated sugarcane setts. Pesquisa Agropecuária Tropical, Goiânia, v. 49, e55132, 2019. Disponível em: https://www.revistas.ufg.br/pat/article/view/55132/33350.