Use este identificador para citar ou linkar para este item: http://repositorio.bc.ufg.br/handle/ri/18217
Tipo do documento: Artigo
Título: Subcellular localization of myosin-V in the B16 melanoma cells, a wild-type cell line for the dilute gene
Autor: Nascimento, Alexandra Aparecida da Cunha
Amaral, Rita Goreti
Bizario, João Carlos da Silva
Larson, Roy Edward
Espreafico, Enilza Maria
Abstract: The discovery that the dilute gene encodes a class V myosin led to the hypothesis that this molecular motor is involved in melanosome transport and/or dendrite outgrowth in mammalian melanocytes. The present studies were undertaken to gain insight into the subcellular distribution of myosin-V in the melanoma cell line B16-F10, which is wild- type for the dilute gene. Immunofluorescence studies showed some degree of superim- posed labeling of myosin-V with melanosomes that predominated at the cell periphery. A subcellular fraction highly enriched in melanosomes was also enriched in myosin-V based on Western blot analysis. Immunoelectron microscopy showed myosin-V labeling associated with melanosomes and other organelles. The stimulation of B16 cells with the a -melanocyte-stimulating hormone led to a significant increase in myosin-V expression. This is the first evidence that a cAMP signaling pathway might regulate the dilute gene expression. Immunofluorescence also showed an intense labeling of myosin-V indepen- dent of melanosomes that was observed within the dendrites and at the perinuclear region. Although the results presented herein are consistent with the hypothesis that myosin-V might act as a motor for melanosome translocation, they also suggest a broader cytoplasmic function for myosin-V, acting on other types of organelles or in cytoskeletal dynamics.
País: Estados unidos
Unidade acadêmica: Faculdade de Farmácia - FF (RG)
Citação: NASCIMENTO, Alexandra A. C. et al . Subcellular localization of myosin-V in the B16 melanoma cells, a wild-type cell line for the dilute gene. Molecular Biology of the Cell, Bethesda, v. 8, n. 10, p. 1971-1988, 1997.
Tipo de acesso: Acesso Aberto
Identificador do documento: 10.1091/mbc.8.10.1971
Identificador do documento: 10.1091/mbc.8.10.1971
URI: http://repositorio.bc.ufg.br/handle/ri/18217
Data de publicação: Out-1997
Aparece nas coleções:FM - Artigos publicados em periódicos

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