Use este identificador para citar ou linkar para este item: http://repositorio.bc.ufg.br/handle/ri/18610
Tipo do documento: Artigo
Título: Production and characterization of tyrosinase activity in Pcnoporus sanguineus CCT-4518 crude extract
Autor: Duarte, Lívia Teixeira
Tiba, Joyce Batista
Santiago, Mariângela Fontes
Garcia, Telma Alves
Bara, Maria Teresa Freitas
Abstract: Tyrosinase is an enzyme of industrial interest. The production and characterization of tyrosinase from P. sanguineus CCT-4518 were investigated. The selection of inductors, luminosity influence, inoculum size and type of culture medium on the production of tyrosinase and the effect of inhibitors on enzyme activity were performed. Optimum conditions for intracellular tyrosinase production was observed after 2 days using 0.15% L-tyrosine as inducer, in the presence of light, with inoculum size of 10 mycelium discs, using 2% malt extract broth medium, incubated at 30°C, and constant agitation of 150 rpm. Tyrosinase activity was completely inhibited by the addition of 6 mM salicylhydroxamic acid or phenylthiourea, however an inhibition of 4.15% was recorded by the addition of 0.1 mM sodium azide. No inhibition could be detected in case of 0.1 mM phenyl methanesulfonyl fluoride addition. Optimal conditions for intracellular tyrosinase activity using L-dopa as substrate were observed at pH 6.6 and 45°C. Thermal stability studies indicated that the enzyme is stable at 45°C for 15 minutes. Higher temperatures decreased tyrosinase activity. Enzyme production was confirmed by non-denaturing polyacrylamide gel electrophoresis and the protein profile was investigated by denaturing polyacrylamide gel electrophoresis.
Palavras-chave: Pycnoporus sanguineus
White-rot fungi
Tyrosinase
Biotechnology
MBTH
País: Brasil
Unidade acadêmica: Faculdade de Farmácia - FF (RG)
Citação: Duarte, Lívia Teixeira; TIBA, Joyce Batista; SANTIAGO, Mariangela Fontes; GARCIA, Telma Alves; BARA, Maria Teresa Freitas. Production and characterization of tyrosinase activity in Pcnoporus sanguineus CCT-4518 crude extract. Brazilian Journal of Microbiology, São Paulo, v. 43, p. 21-29, 2012.
Tipo de acesso: Acesso Aberto
Identificador do documento: 10.1590/S1517-83822012000100003 
Identificador do documento: 10.1590/S1517-83822012000100003 
URI: http://repositorio.bc.ufg.br/handle/ri/18610
Data de publicação: 2012
Aparece nas coleções:FF - Artigos publicados em periódicos

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