Phylogenetic characterization of Babesia canis vogeli in dogs in the state of Goiás, Brazil
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2011-12
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Resumo
The genus Babesia comprises protozoa that cause diseases known as babesiosis. Dogs are commonly affected by
Babesia canis or Babesia gibsoni. Babesia canis is divided into the subspecies Babesia canis canis, Babesia canis vogeli
and Babesia canis rossi. Among these, Babesia canis vogeli predominates in Brazil. The objective of this study was
to conduct a phylogenetic analysis on Babesia isolates from dogs in Goiânia, Goiás. Blood samples were obtained
from 890 dogs presenting clinical signs suggestive of canine babesiosis that were attended at a veterinary hospital of
Goiás. Only samples presenting typical intraerythrocytic parasites were used in the study. These were subjected to DNA
extraction and amplification of a fragment of the 18S rRNA, by means of PCR. The PCR products were purified and
sequenced. Sequences were obtained from 35 samples but only 17 of these were kept after quality assessment. Similarity
analysis using BLASTn demonstrated that all 17 sequences corresponded to B. canis vogeli. Analysis using the Mega4
software showed that the isolates of B. canis vogeli from dogs in Goiânia present a high degree of molecular similarity
(99.2 to 100%) in comparison with other reference isolates from other regions of Brazil and worldwide, deposited in
GenBank.
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Babesia canis vogeli, Canine babesiosis, Molecular characterization, Phylogeny, Babesia canis vogeli, Filogenia, Babesiose canina, Caracterização molecular
Citação
DUARTE, Sabrina Castilho et al. Phylogenetic characterization of Babesia canis vogeli in dogs in the state of Goiás, Brazil. Revista Brasileira de Parasitologia Veterinária, Jaboticabal, v. 20, n. 4, p. 274-280, 2011.