Use este identificador para citar ou linkar para este item: http://repositorio.bc.ufg.br/handle/ri/18673
Tipo do documento: Artigo
Título: Assessment of genetic variability within and among coffee progenies and cultivars using RAPD markers
Autor: Silveira, Sheila Recepute
Ruas, Paulo Maurício
Ruas, Claudete de Fátima
Sera, Tumoru
Carvalho, Valdemar de Paula
Coelho, Alexandre Siqueira Guedes
Abstract: The RAPD technique associated with restriction digestion of genomic DNA was used to assess the genetic variability within and among nine populations of Coffea arabica, including six progenies belonging to the Sarchimor germplasm, the progeny PR 77054-40-10 (Catuaí Vermelho IAC 81 x Icatu), and two commercial cultivars (IAPAR 59 and Catuaí Vermelho IAC-81). These populations were evaluated using analysis of molecular variance (AMOVA), genetic similarity among progenies, and percentage of polymorphic loci. A total of 99 RAPD markers were evaluated of which 67 (67.67%) were polymorphic. AMOVA showed that 38.5% and 61.5% of the genetic variation was distributed among and within populations, respectively. The fixation index (FST) of the genotypes was 0.385. The mean genetic variability estimated within populations ranged from 15.58 (IAPAR 59) to 8.27 (Catuaí Vermelho IAC 81). A distinct level of genetic variability was revealed for each of the coffee progenies and varieties studied. The methodology used in this investigation was useful to determine the genetic variability within and among C. arabica L. populations providing significant information for coffee breeding.
Palavras-chave: Coffee breeding
Genetic variability
Molecular variance
RAPD markers
País: Brasil
Unidade acadêmica: Escola de Agronomia - EA (RG)
Citação: SILVEIRA, Sheila Recepute et al. Assessment of genetic variability within and among coffee progenies and cultivars using RAPD markers. Genetics and Molecular Biology, Ribeirão Preto, v. 26, n.3, p. 329-336, 2003.
Tipo de acesso: Acesso Aberto
Identificador do documento: 10.1590/S1415-47572003000300018
Identificador do documento: 10.1590/S1415-47572003000300018
URI: http://repositorio.bc.ufg.br/handle/ri/18673
Data de publicação: 2003
Aparece nas coleções:EA - Artigos publicados em periódicos

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