Use este identificador para citar ou linkar para este item: http://repositorio.bc.ufg.br/handle/ri/19139
Tipo do documento: Artigo
Título: Acomparison of cell disruption procedures for the recovery of intracellular carotenoids from Sporobolomyces ruberrimus H110
Autor: Pennacchi, Mariana Gomes Clemente
Rodríguez Fernández, Daniel Ernesto
Vendruscolo, Francielo
Maranho, Leila Teresinha
Marc, Ivan
Cardoso, Lígia Alves da Costa
Abstract: Carotenoids are intracellular pigments produced by microorganisms, including some species of yeasts, in the stationary phase of growth by the secondary metabolic pathways. In the present study, different methods of Sporobolomyces ruberrimus H110 yeast cell lysis were evaluated with the objective of optimizing the recovery of intracellular pigments. Three extraction methods were used: vortex (glass beads and quartz stones), planetary mill (glass beads and quartz stones) and dimethyl sulfoxide. For each one of the lysis agents studied, factorial designs were developed considering as independent variables the agitation speed and lysis agent concentration. A central composite planning was defined considering as independent variables the lysis agent concentration and agitation speed, analyzing as response the estimated total number of extracted carotenoids. From the methods studied, a better extraction of total carotenoid (1.74 mg.g-1 of cells and of 1.57 mg.g-1 of cells) using the planetary mill method with 135 mg of glass beads or irregular quartz stones, with an agitation speed of 200 rpm. As to the cell lysis, the analysis indicated that the mechanical methods studied showed to be efficient in regards to cell laceration.
Palavras-chave: Yeast
Carotenoids
Cell lysis
Extraction
Planetary mill
País: Estados unidos
Unidade acadêmica: Escola de Agronomia - EA (RG)
Citação: PENNACCHI, Mariana Gomes Clemente et al. Acomparison of cell disruption procedures for the recovery of intracellular carotenoids from Sporobolomyces ruberrimus H110. International Journal of Applied Biology and Pharmaceutical Technology, Houston, v. 6, n. 1, p. 136-143, 2015.
Tipo de acesso: Acesso Aberto
Endereço da licença: http://creativecommons.org/licenses/by-nc-nd/3.0/br/
URI: http://repositorio.bc.ufg.br/handle/ri/19139
Data de publicação: Mar-2015
Aparece nas coleções:EA - Artigos publicados em periódicos

Arquivos associados a este item:
Arquivo Descrição TamanhoFormato 
Artigo - Mariana Gomes Clemente Pennacchi - 2015.pdf473,97 kBAdobe PDFThumbnail
Baixar/Abrir


Este item está licenciada sob uma Licença Creative Commons Creative Commons