Use este identificador para citar ou linkar para este item: http://repositorio.bc.ufg.br/handle/ri/19143
Tipo do documento: Artigo
Título: Sequence analysis and characterization of rolling-circle replicating plasmid pVCM01 from Salmonella enterica
Autor: Troian, Ana Flavia Batista Penido
Mendes, Paulo Vinicius da Costa
Campos, Ivan Torres Nicolau de
Bataus, Luiz Artur Mendes
Abstract: Aims: Characterization of cryptic plasmid pVCM01 (accession number JX133088) isolated from Salmonella enterica Enteritidis. Methodology and results: The complete sequence of pVCM01 was obtained. This plasmid possesses 1981 bp, with G+C content of 57% in agreement of the range of Salmonella genomic DNA. pVCM01 has a high degree of similarity to pB and pJ plasmids. It possesses six main open reading frames, only one have a very high degree of amino acid identity with protein involved in the rolling-circle-like replication (RCR). Based on the sequence similarities, pVCM01 plasmid belonged to the pC194/pUB110 rolling-circle replicating plasmid family. The Rep pVCM01 possesses the motifs: FLTLTVRN, HPHFHTL, SGDGYVKHERW, which were present in all Rep proteins. Conclusion, significance and impact of study: The small size of pVCM01 plasmid and its stability in E. coli cells, make it an attractive candidate to develop new vectors, such as cloning and/or expression vector.
Palavras-chave: Salmonella enterica
Rep protein
Rolling-circle replicating plasmid
País: Outros
Unidade acadêmica: Instituto de Ciências Biológicas - ICB (RG)
Citação: PENIDO, Ana Flávia Batista et al. Sequence analysis and characterization of rolling-circle replicating plasmid pVCM01 from Salmonella enterica. Malaysian Journal of Microbiology, Penang, v. 9, n. 4, p. 331-334, 2013.
Tipo de acesso: Acesso Aberto
Identificador do documento: 10.21161/mjm.50913
Endereço da licença: http://creativecommons.org/licenses/by-nc-nd/3.0/us/
Identificador do documento: 10.21161/mjm.50913
URI: http://repositorio.bc.ufg.br/handle/ri/19143
Data de publicação: 2013
Aparece nas coleções:EA - Artigos publicados em periódicos

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