Estabelecimento in vitro de duas espécies de bambu: Dendrocalamus asper (Schultes f.) Backer ex Heyne e Bambusa oldhamii Munro
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Data
2015
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Resumo
Bamboo has economic importance in many areas such as construction,
manufacturing paper, energy supply, food industry, furniture, etc. Getting seedling is
the main difficulty for the large-scale cultivation, in vitro culture becomes the most
appropriate tool to resolve the issue. Several protocols have been established for
many species of bamboo. However, the known protocols are not reproducible for
different species. The aim was development of protocol for in vitro establishment and
multiplication of two species with economic importance, Dendrocalamus asper
(Schultes f.) Backer ex Heyne e Bambusa oldhamii Munro, to eliminate or reduce the
microorganism that affect cultivation and in vitro multiplication. Decontamination
methods were tested with or without antibiotic and the cytokinins effect on explant
shoots from nodal segments on MS medium (MURASHIGE e SKOOG, 1962). The
percentage of decontamination of explants subjected to aseptic process varied
between the two species analyzed. Explants of D. asper had higher percentage of
contamination. By comparing the percentage of contamination between the two
treatments B. oldhamii, with or without antibiotic, was finding that use of streptomycin
was effective in reducing 38.05% of bacterial contamination. Although the use of
streptomycin does not interfere in the number of issued shoots, new experiments
should be done to check the increase in the fungal contamination found during the
experiments.
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Palavras-chave
Cultura de tecidos, Micropropagation, Micropropagação, Produção de mudas, Seedling production, Tissue culture
Citação
ARAUJO, Cris Hanny Pires et al. Estabelecimento in vitro de duas espécies de bambu: Dendrocalamus asper (Schultes f.) Backer ex Heyne e Bambusa oldhamii Munro. Enciclopédia Biosfera, Goiânia, v. 11, n. 22, p. 1172-1182, 2015.