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Tipo do documento: Artigo
Título: Immunogenicity of a recombinant Mycobacterium smegmatis vaccine expressing the fusion protein CMX in cattle from Goiás state, Brazil
Autor: Silva, Duanne Alves da
Cavalcanti, Marcos Antonio Rocha
Oliveira, Fábio Muniz de
Trentini, Monalisa Martins
Junqueira-Kipnis, Ana Paula
Kipnis, Andre
Abstract: This study aimed to evaluate the immunogenicity of a recombinant Mycobacterium smegmatis vaccine expressing the CMX fusion protein composed of immunodominant epitopes Ag85C, MPT51 and HspX of Mycobacterium tuberculosis, which are important mycobacteria virulence factors. A group of Nelore heifers that were 10 to 12 months of age and negative for the tuberculin skin test (TST) were immunized with four doses of the recombinant vaccine mc2-CMX (M. smegmatis-Ag85C-MPT51-HspX) during a period of one year. Before each immunization, blood was collected to obtain sera for antibody analysis. Serological analysis demonstrated that mc2-CMX was able to induce a humoral response with increased levels of specific IgG antibodies against CMX, despite minimum antibody levels being detected for individual Ag85C, MPT51 or HspX recombinant antigens. However, there was no significant increase in specific CD4+ IFN-γ-positive T cells. Lymphadenomegaly was observed in superficial cervical lymph nodes adjacent to the site of vaccination among mc2-CMX-vaccinated bovines, and the histopathological analysis demonstrated follicular hyperplasia without inflammatory infiltrate or granuloma formation. Animals remained negative for the TST until the end of the experiments, showing no cross-reactivity with the recombinant vaccine and tuberculin proteins. We discuss the potential of mc2-CMX to induce an immune response in cattle.
Palavras-chave: Environmental mycobacteria
Immunological response
Public health
Mycobacterium bovis
País: Outros
Unidade acadêmica: Instituto de Patologia Tropical e Saúde Pública - IPTSP (RG)
Citação: SILVA, Duanne Alves da et al. Immunogenicity of a recombinant Mycobacterium smegmatis vaccine expressing the fusion protein CMX in cattle from Goiás state, Brazil. Journal of Veterinary Medical Science, Tokyo, v. 76, n. 7, p. 977-984, 2014.
Tipo de acesso: Acesso Aberto
Identificador do documento: 10.1292/jvms.13-0338
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Identificador do documento: 10.1292/jvms.13-0338
Data de publicação: 2014
Aparece nas coleções:IPTSP - Artigos publicados em periódicos

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