Use este identificador para citar ou linkar para este item: http://repositorio.bc.ufg.br/handle/ri/19340
Tipo do documento: Artigo
Título: Generation of recombinant bacillus Calmette–Guérin and Mycobacterium smegmatis expressing BfpA and intimin as vaccine vectors against enteropathogenic Escherichia coli
Autor: Vasconcellos, Halyka Luzorio Franzotti
Scaramuzzia, Karina
Nascimento, Ivan Pereira
Ferreira Junior, Jorge Mario da Costa
Abe, Cecilia Mari
Piazza, Roxane Maria Fontes
Kipnis, Andre
Silva, Wilmar Dias da
Abstract: Enteropathogenic Escherichia coli (EPEC) is an important cause of diarrhea in children. EPEC adheres to the intestinal epithelium and causes attaching and effacing (A/E) lesions. Recombinant Mycobacterium smegmatis (Smeg) and Mycobacterium bovis BCG strains were constructed to express either BfpA or intimin. The entire bfpA gene and a portion of the intimin gene were amplified by PCR from EPEC genomic DNA and inserted into the pMIP12 vector at the BamHI/KpnI sites. The pMIP bfpA and pMIP intimin vectors were introduced separately into Smeg and BCG. Recombinant clones were selected based on kanamycin resistance and designated rSmeg pMIP (bfpA or intimin) and rBCG pMIP (bfpA or intimin). The expression of bfpA and intimin was detected by Immunoblotting using polyclonal anti-BfpA and anti-intimin antibodies. The immunogenicity of these proteins was assessed in C57BL/6 mice by assaying the feces and serum for the presence of anti-BfpA and anti-intimin IgA and IgG antibodies. TNF- and INF- were produced in vitro by spleen cells from mice immunized with recombinant BfpA, whereas TNF- was produced in mice immunized with recombinant intimin. The adhesion of EPEC (E2348/69) to HEp-2 target cells was blocked by IgA or IgG antibodies from mice immunized with recombinant BfpA or intimin but not by antibodies from non-immunized mice. Immunogenic non-infectious vectors containing relevant EPEC virulence genes may be promising vaccine candidates.
Palavras-chave: Diarrhea
EPEC
BCG
M. smegmatis
Recombinant vaccine
bfpA and intimin
País: Holanda
Unidade acadêmica: Instituto de Patologia Tropical e Saúde Pública - IPTSP (RG)
Citação: VASCONCELLOS, Halyka Luzorio Franzotti et al. Generation of recombinant bacillus Calmette Guérin and Mycobacterium smegmatis expressing BfpA and intimin as vaccine vectors against enteropathogenic Escherichia coli. Vaccine, Amsterdam, v. 30, n. 41, p. 5999-6005, 2012.  
Tipo de acesso: Acesso Aberto
Identificador do documento: 10.1016/j.vaccine.2012.05.083
Endereço da licença: http://creativecommons.org/licenses/by-nc-nd/4.0/
Identificador do documento: 10.1016/j.vaccine.2012.05.083
URI: http://repositorio.bc.ufg.br/handle/ri/19340
Data de publicação: 2012
Aparece nas coleções:IPTSP - Artigos publicados em periódicos

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