New vectors derives from pUC 18 for clonig and thermal-induced expression in Escherichia coli
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Data
2009
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Resumo
We report the construction of two vectors for Escherichia coli: pUC72, for molecular cloning, and pPLT7,
for thermal-induced expression. The main feature of pUC72 is a novel polylinker region that includes
restriction sites for Nde I and Nco I which provide an ATG codon for proper translation initiation of
expressed genes. Vector pPLT7 is ideal for thermo-inducible expression in host cells that carry the cI857
repressor gene. The use of pPLT7 was validated by the successful expression of the genes encoding carp
and porcine growth hormones. These vectors provide novel cloning possibilities in addition to simple,
non-expensive, high level expression of recombinant proteins in E. coli.
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Palavras-chave
Heterologous expression, Induced expression., Escherichia coli, Molecular cloning
Citação
XAVIER, Mauro Aparecido Souza et al. New vectors derives from pUC 18 for clonig and thermal-induced expression in Escherichia coli. Brazilian Journal of Microbiology, São Paulo, v. 40, n. 4, p. 778-781, 2009.