Avaliação da citotoxicidade e internalização celular in vitro de nanopartículas poliméricas contendo melatonina em células de glioblastoma multiforme U87MG
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Data
2020-12-09
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Universidade Federal de Goiás
Resumo
Glioblastoma multiforme (GBM) is a malignant primary brain tumor, with low prognosis profile. Moreover, GBM is responsible for about 4% of cancer-related deaths. Currently, the treatment of GBM consists on surgical removal of the tumor followed by radiotherapy and chemotherapy. It has been reported in the literature the antitumor activity of melato- nin (MLT) for the treatment of GBM. In addition, the use of nanoparticles, as drug carries, have improved the targeting of drugs to the central nervous system. This study aimed to evaluate the in vitro cytotoxicity and cellular uptake of melatonin-loaded polymeric nano- particles in human GBM cell line (U87MG). The cytotoxicity of formulation was evaluated by the MTT assay. The cellular uptake of nanoparticles was observed by fluorescence microscopy and flow citometry. The results noticed that MLT demonstrated a selective cytotoxic activity against U87MG cells when encapsulated in the particles. These results may be justified by the facilitated cellular internalization of the nanoparticles and the re- lease of MLT over time. In conclusion, the nanoencapsulation was crucial for the selective cytotoxic activity of MLT in U87MG, representing a promising strategy for the treatment of GBM.
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Citometria de fluxo, Microscopia de fluorescência, MTT, Tumor cerebral., Brain tumor, Flow cytometry, Fluorescence microscopy, MTT assay
Citação
SALOMÃO, Mariana Arraes. Avaliação da citotoxicidade e internalização celular in vitro de nanopartículas poliméricas contendo melatonina em células de glioblastoma multiforme U87MG. 2020. 30 f. Trabalho de Conclusão de Curso (Graduação) – Faculdade de Farmácia, Universidade Federal de Goiás, Goiânia, 2020.