DNA metabarcoding reveals the responses of prokaryotes and eukaryotes microbiota to warming: are the patterns similar between taxonomic and trophic groups?
Nenhuma Miniatura disponível
Data
2020-08
Título da Revista
ISSN da Revista
Título de Volume
Editor
Resumo
The aquatic microbiota is involved in crucial ecosystem functions. Thus, investigating the effects of global
warming on these organisms is highly relevant, especially given the numerous climatic changes expected by the
end of the century. In this study, we used an experimental approach and high-throughput sequencing to evaluate
the shortterm effect of warming predicted by different future scenarios in the composition of the planktonic
freshwater bacteria and microeukaryotes, and to verify if the same effects occur for each trophic level separately
(autotrophic, heterotrophic and mixotrophic). Our experiment demonstrated that the composition for eukaryotes
and prokaryotes based on DNA metabarcoding is affected by the increase in temperature and these have a similar
pattern of response to warming. This highlights the temperature importance in structuring the communities of
different groups. Modifications in the communities were observed through the substitution of specific taxa,
which occurred mainly in warmer levels. Changes in community composition were also identified when trophic
levels were assessed separately. Mixotrophic eukaryotes organisms are more sensitive to warming, modifying the
patterns of composition with an increase in temperature of 2 °C. Microeukaryotes and heterotrophic bacteria
were more resistant, with alterations in the communities composition visualized only in higher warming levels.
The composition of autotrophic organisms was not affected by the increase in water temperature in any of the
biological classifications evaluated, although the richness of eukaryotic autotrophic has decreased with
warming. Our results contribute to predict how different biological levels and trophic groups of the aquatic
microbiota respond to global warming. This approach is relevant because warming leads to changes in community
composition and affects ecosystem processes essentials to the aquatic environment.
Descrição
Palavras-chave
Bacterioplankton, 16S rDNA, 18S rDNA, Protists
Citação
MACHADO, Karine Borges; ANTUNES, Adriana Maria; TARGUETA, Cíntia Pelegrineti; FERNANDES, Jordana Gontijo; SOARES, Thannya Nascimento; NABOUT, João Carlos. DNA metabarcoding reveals the responses of prokaryotes and eukaryotes microbiota to warming: are the patterns similar between taxonomic and trophic groups?. Ecological Indicators, Oxford, v. 115, p. 106452, Aug. 2020. DOI: 10.1016/j.ecolind.2020.106452. Disponível em: https://www.sciencedirect.com/science/article/pii/S1470160X20303897. Acesso em: 24 mar. 2023.