Programa de Pós-graduação em Ciência Animal
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Navegando Programa de Pós-graduação em Ciência Animal por Por Orientador "Arnhold, Emmanuel"
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Item Caracterização e criopreservação de sêmen de tambaqui (Colossoma macropomum) em diferentes crioprotetores(Universidade Federal de Goiás, 2014-03-10) Lenz, Dayane Regina; Meirinhos, Maria Lúcia Gambarini; Paula, Fernanda Gomes de; Arnhold, Emmanuel; http://lattes.cnpq.br/7156945506134934; Arnhold, Emmanuel; Reidel, Adilson; Lima, Milton Luiz MoreiraThe objective of the study is to characterize the semen of tambaqui Colossoma macropomum during the breeding season and to evaluate the efficiency of four cryoprotectants added to the extender on sperm cryopreservation of tambaqui and to define the best protocol for thawing. Three semen samples were collected at regular intervals from ten males (pool). In the first stage of the experiment the characterization of semen in natura was performed and it was subjected to the routine analysis in optical microscopy: motility, vigor, life time and counting the number of spermatozoa. In the second stage of the experiment cryopreservation of semen was performed. The cryoprotectants glycerol, dimethylsulfoxide (DMSO), dimethylformamide (DMF) and methanol were tested at a ratio of 10.0%. For the assessment of viability and comparison of treatments testing of post-thaw samples in vivo was performed in three different protocols for each cryoprotectant, in thawing time 15, 30 and 45 seconds, 30°C temperature, totalizing 12 treatments with five replicates each. Quantitative variables were subjected to analysis of variance (ANOVA) and Tukey test while qualitative variables were subjected to the Freedman test with 0.05 significance. No significant differences (p> 0.05) were observed for most of the evaluated parameters. The sperm concentration was higher in the first sampling (14.54 billion sperm /mL) and the lifetime decreased at the end of the experiment. The cryoprotectant medium containing methanol and thawing for 30 s was the treatment that showed the best results fertilization (77.65%/).Item Desenvolvimento embrionário e larval de Colossoma macropomum, Piaractus brachypomus e do híbrido tambatinga(Universidade Federal de Goiás, 2014-08-28) Lima, Mayanny Carla de Carvalho; Meirinhos, Maria Lúcia Gambarini; Paula, Fernanda Gomes de; Arnhold, Emmanuel; http://lattes.cnpq.br/7156945506134934; Arnhold, Emmanuel; Oliveira, Raquel Priscila de Castro; Onã, Cely Marine Melo eKnowing fish embryonic and larval development is essential to understand species biology, supply chain development and studies of breeding and reproduction of fish technology. This study aimed was to describe Tambaqui (Colossoma macropomum), pirapitinga (Piaractus brachypomus) and its hybrid tambatinga (♀C. Macropomum x ♂ P. brachypomus). embryonic and larval development. The experiment was conducted in Buritizal Fish Farm, located in Palmeiras de Goiás district. It was used three Tambaqui females, three pirapitinga females, five males tambaqui and five pirapitinga in reproductive age. Reproduction was performed by hormonal induction. Eggs were incubated in vertical incubators of 200L water capacity. Sampling took place at pre-determined time from the moment of extrusion of oocytes to 84 hours after fertilization. Samples were fixed in formalin buffered saline to 10% immediately after collection, and examined under a stereomicroscope. Tambaqui and pirapitinga females produced spherical, non-adhesive, translucent oocytes. During embryonic development stage of the zygote, cleavage, morula, blastula, gastrula, organogenesis and hatching were observed. The vegetative pole remained undivided throughout embryonic development, characterizing segmentation type meroblastic discoidal, typical egg yolk with large amounts. Pirapitinga and tambaqui eggs hatched 15 hours or 422 hours-degree after fertilization, tambatinga hybrid eggs hatched 14 hours or 394 hours-degree after fertilization, at an average temperature of 28.13 ± 0,06ºC. Parental species and hybrid under study showed characteristic embryonic and larval of telolecithal fish with eggs, wherein the newly hatched larvae, has not yet fully formed, all organic systems, limited to a few organs, and depending on the yolk for nourishment . No morphological difference in embryonic and larval development of hybrid tambatinga regarding parental species was observed.Item Resultados produtivos no incubatório e na granja de frangos de corte utilizando sistema de incubação em estágio múltiplo e estágio único(Universidade Federal de Goiás, 2013-04-02) Mesquita, Mariana Alves; Gonzales, Elisabeth; http://lattes.cnpq.br/5436760646498130; Leandro, Nadja Susana Mogyca; http://lattes.cnpq.br/3136809931691012; Arnhold, Emmanuel; http://lattes.cnpq.br/7156945506134934The aim of this study was to compare the incubation systems in multiple stage and single stage, by evaluating the performance of the incubation process and the production performance of broilers originated from embryonated eggs incubated in the both systems. To evaluate the yield of incubation five experiments were performed, using eggs of Cobb broiler breeders. Each test was divided into two phases. In the first experimental phase, eggs weight, chicks weight, residual yolk weight, length and quality score of the chicks were analyzed using randomized block design with two treatments (single stage and multiple stage), and with blocks being each experimental trial. In each test, 140 eggs and all the chicks borned from those eggs from each treatment were assessed (experimental unit). In the second phase of the trial, a total of 25.360 eggs and was incubated and spread on trays for analysis of hatchability, hatch window, birth distribution and embryo diagnosis. For hatchability data and hatch window, a randomized block design in a 2x3 factorial arrangement (two incubation systems and three positions of the trays into the incubation machine) was used. The repetitions were composed by the trays, totaling 285. For the results of embryo diagnosis all unhatched eggs from 285 selected trays were evaluated. The productive performance was evaluated until 35 days of age by means of a completely randomized 2x2 factorial arrangement (chicks from two stages of incubation and both sexes), with six replicates. Each experimental unit consisted of 25 birds, totaling 600 birds. We concluded that incubation of fertile eggs from Cobb 500 broiler breeders in single stage machines enables to obtain better productivity of the hatchery than incubation in multiple stage machines, as a result of higher hatchability and better neonate quality. The productive performance of broiler up to 35 days of age was not affected by the type of machine used for incubation.