Encapsulação da genisteína em lipossomas: caracterização das interações farmacolipídeo, estabilidade e atividade antioxidante
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Data
2008-04-28
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Universidade Federal de Goiás
Resumo
Genistein is the most abundant isoflavone, displaying the largest
number of biological activities. Most studies on genistein have been based on
its antioxidant, estrogenic, tyrosine kinase and carcinogenesis inhibitor
activities. Genistein is practically insoluble in water and oil in the active form
of aglicone, which presents a technical difficulty. To circumvent this problem,
the encapsulation of genistein in phosphatidylcholine liposomes seems an
efficient alternative for the improvement of solubility. In this work, genistein
was encapsulated in phosphatidylcholine liposomes and the drugphospholipid
interactions were characterized. It was demonstrated that the
interaction of genistein with this lipid membrane model lead to an increased
membrane rigidity caused by a tighter packing of the phospholipid bilayer.
Encapsulation efficiency for genistein in liposomes reached 99%, with a
maximum loading efficiency at the molar ratio of 1:3
(genistein:phosphatidylcholine), resulting in an increase of 1000 times in the
water solubility of genistein. Genistein was able to protect membrane
phospholipids against CuSO4 induced peroxidation. The liposomal
preparation remained stable for at least 40 days, showing its potential as a
nanostructured delivery system for this isoflavone for therapeutic and
cosmetic applications.
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RIBEIRO, Lidiane Radoll. Genistein encapsulation into liposomes: characterization of drug-lipid interactions, stability and antioxidant activity. 2008. 15 f. Dissertação (Mestrado em Educação em Química) - Universidade Federal de Goiás, Goiania, 2008.