Antioxidante na viabilidade do sêmen equino congelado e refrigerado
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2011-04-18
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Universidade Federal de Goiás
Resumo
In the equine world is increasing the use of frozen semen and
cooled due to the recent release of its use for most of
breeders' associations. However, the fertility of frozen semen is still low and this hinders their widespread use. The objective was to evaluate the effect In vitro addition of glutathione on sperm concentrations in five of 12 stallions in different protocols of freezing, and I Experiment (EI): automated freezing immediately after harvest and Experiment II (EII): automated freezing (MRI) and manual (RG) after passive cooling 24 h at 16 ° C. The variables assessed were total and progressive motility, vigor, plasma membrane integrity and acrosomal. The addition of glutathione in concentration of 2.5 mM in the midst of freezing preserves the total motility, significantly increases the motility and integrity of plasma membrane, both for semen frozen immediately after harvesting and for the refrigerated and frozen manually. Concentrations exceeding 2.5 mM were deleterious to sperm in different freezing protocols, with or without passive cooling. In chapter 3 aimed to evaluate the in vitro effect of the addition of cysteine ​​and glutathione in seven
treatments on sperm from 12 stallions in protocol passive cooling to 16 ° C for 36h. The variables assessed were total motility and progressive force and plasma membrane integrity and acrosomal in seven different times (0, 6, 12, 18, ​​24, 30 and 36h). For discussion of data were taken into account only the values ​​of the moments 1, 3, 5 and 7 (0, 12, 24 and 36h). The concentration of 1.0 mM cysteine ​​was more effective in protection of sperm cells in the trading system of the passive cooling 16 º C for 36h, with higher values ​​of motility and membrane integrity.
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OLIVEIRA, Rodrigo Arruda de. Antioxidants for visiability of equine frozen and cooled semen. 2011. 54 f. Tese (Doutorado em Ciências Agrárias) - Universidade Federal de Goiás, Goiânia, 2011.