Construção e análise de uma biblioteca de cDNA de Trichoderma harzianum crescido na presença de parede celular de Fusarium solani
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2010-02-26
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Universidade Federal de Goiás
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Species of Trichoderma are commercially applied as biological control agents against plant fungal pathogens. Their action is based on a set of events, such as the production of antifungal metabolites, competition for space and nutrients and mycoparasitism. However the biocontrol mechanism of the interaction between Trichoderma harzianum and Fusarium solani is not yet understanded at the transcriptome level. This study aimed to initiate the preliminary development of an expressed sequence tag (EST) database for Trichoderma harzianum and thereby gain potentially useful information on
Trichoderma gene sequences in order to elucidate the integrated biocontrol mechanism. Partial sequencing of anonymous cDNA clones is a widely used technique for gene identification. A directional cDNA library has been
constructed from mycelium of Trichoderma harzianum grown on cell wall isolated from Fusarium solani. 3984 clones have been randomly selected, subjected to single-pass sequencing from the 5 end of the vector, and identified by sequence similarity searches against gene sequences in GenBank fungal database. Of the 3984 mycelium clones, 40.8% exhibit similarity to known genes. Analysis of the identified clones indicated sequence similarity to a broad diversity of genes encoding proteins such as enzymes, structural proteins, and regulatory factors. A significant proportion of genes identified were involved in processes related to mycoparasitism (3,81%), as would be expected in biocontrol fungus. These results present the successful application of EST analysis in the interaction of Trichoderma harzianum and Fusarium solani to provide a preliminary indication of gene expression.
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STEINDORFF, Andrei Stecca. Construction and analisys of a cDNA library of Trichoderma harzianum grown in presence of Fusarium solani cell wall. 2010. 71 f. Dissertação (Mestrado em Ciências Biolóicas) - Universidade Federal de Goiás, Goiânia, 2010.