Caracterização da expressão de inibidores de poligalacturonases (PGIPs) em resposta aos estresses biótico e abiótico em plantas de feijão comum (Phaseolus vulgaris L.)

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2011-11-26

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Universidade Federal de Goiás

Resumo

Sclerotinia sclerotiorum (Lib.) de Bary is a necrotrophic fungal pathogen that causes the disease known as white mold in many plants. During infection, it secretes several endopolygalacturonases (PGs) to degrade cell wall pectin. To counteract the action of PGs, plants express polygalacturonases-inhibiting proteins (PGIPs) that reduce the hydrolytic activity of endo-PGs and favor the accumulation of oligogalacturonides (OGs) with are elicitors of a variety of defense responses. PGIPs belong to the superfamily of leucine rich repeat (LRR) proteins and play important roles in resistance to infection of pathogens. In this study, real time RT-PCR was used to evaluate the expression of Pvpgip genes in dry bean plants (Phaseolus vulgaris L) submitted to different stress conditions. Transcriptional analysis showed that these genes are differentially expressed and activated by biotic (S. sclerotiorum infection) and abiotic (wound or methyl jasmonate treatment) stresses. Pvpgip1 was induced at early stages of the infection especially for plants treated with methyl jasmonate (MeJA) in which the transcript accumulation was higher. High levels of Pvpgip2 and Pvpgip3 expression were observed in infect plants treated or not with MeJA. All treatments showed induction of gene Pvpgip4. These results show that the/ four genes Pvpgip respond differently to treatment with the resistance inducer, fungal infection and wound.

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OLIVEIRA, Marília Barros. Characterization of the expression of polygalacturonase inhibitors (PGIPs) in response to biotic and abiotic stresses in plants of common bean (Phaseolus vulgaris L.). 2011. 85 f. Dissertação (Mestrado em Ciências Biolóicas) - Universidade Federal de Goiás, Goiânia, 2011.