Isolamento, seleção e cultivo de bactérias Produtoras de lipases para tratamento de Efluentes da indústria de alimentos
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2012-03-12
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Universidade Federal de Goiás
Resumo
The present work was to isolate, select and cultivate microorganisms that produce
lipases to apply the wastewater treatment industry of potato chips. For the isolation of
microorganisms was used for industrial effluent and sewage treatment plant. The
selection of the strain producing lipase was performed on solid medium containing
tributyrin and in liquid medium, with p-nitrofenolpalmitato. Optimization of lipase
production was performed as variables, the concentration of corn steep liquor,
soybean oil and different mineral sources. The enzyme extract and the commercial
enzyme selected strain were characterized on the optimal activity and stability at
different temperatures and pH values and determined the molecular weight of the
commercial enzyme. For the treatment of sewage, it has been characterized and has
been the optimization of hydrolysis of triglycerides of the effluent having as variables,
pH, enzyme concentration, emulsifier and the influence of fed batch and batch
processes. Twenty-nine were isolated microorganisms were acquired and eleven
research institutes. Of these, four microorganisms were pre-selected for their higher
enzymatic activity. In the selection in submerged fermentation, enzymatic activity was
the best strain of Burkholderia cepacia ATCC 25416. Optimization of lipase
production was obtained with corn steep liquor (13.8 to 26.2 g.L-1) and sodium nitrate
(1.5 to 3.9 g.L-1). In the characterization of the lipase enzyme extract and immobilized
lipase from Burkholderia cepacia, the best results were found at 37ºC and pH
8.0. Were stable at 40°C, and thermosensitive at 50 and 60°C. The inactivation of
lipases template denaturation followed first order. The molecular mass of commercial
enzyme was 33 kDa. For the treatment of effluent, the maximum hydrolytic activity
(0.310 U.mL-1) was observed when the volume of 7% gum arabic was set at 3 mL of
pH between 7.47 to 7.92 and the concentration of enzyme 0.111 to 0.148 g.mL-1. The
mathematical model obtained can be used for batch process and the predictive
largest possible hydrolysis of the triglycerides present in the effluent.
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BUENO, Pabline Rafaella Mello. Isolation, selection and cultivation of bacteria
Producing lipases for treating
Effluents of the food industry. 2012. 123 f. Dissertação (Mestrado em Ciencias Agrárias - Agronomia) - Universidade Federal de Goiás, Goiania, 2012.