Investigação microbiológica e análise qualitativa de achados bacteriológicos e micológicos em placas de cultivo de embriões em laboratório de reprodução humana
Carregando...
Data
2010-08-25
Autores
Título da Revista
ISSN da Revista
Título de Volume
Editor
Universidade Federal de Goiás
Resumo
Introduction: Laboratories in Human Reproduction, quality control is crucial to the success of procedures. The correct implementation of procedures directly influence the results, especially because the vagina, the follicular fluid and semen can not be sterilized. A high degree of hygiene,
cleanliness and disposal of the material must be observed to avoid contamination in the culture media and equipment. The main causes of this contamination has been associated with infection in male and female genital tract and subsequent contamination of oocytes and embryos of patients. Can
also come from contamination of air, machinery and materials such as culture dishes. Objectives: of this study were the presence of market info bacteriological contamination and mycological culture dishes of human embryos and to identify the genus level. Methodology: 125 samples were collected from culture dishes human embryos after transfer to the uterus, in three laboratories of human reproduction in the city of Goiania-GO, in the period 2009 to first half of 2010. The culture media were inoculated in BHI broth and incubated in the greenhouse. Samples that clouded (positivist) were isolated and identified. Results: showed a prevalence of 4.8% of contamination and micro-organisms found were Escherichia coli (50%), Klebsiella sp (16.6%), Pseudomonas sp (16.6%), yeast (16.6%). The E. coli bacteria were of the highest incidence was found in three samples. Although the culture media provide the antibiotics penicillin G (IVF) or gentamicin
(HTF), resistant Gram-negative rods were found. Conclusion: Results with a prevalence of 4.8% of contamination, and the microorganisms isolated and their amount per sample: Escherichia coli (3), Klebsiella sp (1), Pseudomonas sp (1), yeast (1).
Descrição
Palavras-chave
Citação
RIBEIRO, Barbara Rosa Foizer. Microbiological investigation and analysis of qualitative findings
bacteriological and mycological culture plates from embryos in the laboratory of human reproduction. 2010. 101 f. Dissertação (Mestrado em Ciências da Saúde - Medicina) - Universidade Federal de Goiás, Goiânia, 2010.