Desenvolvimento e validação de metodologia por HPLC-PDA para monitoração terapêutica dos níveis plasmáticos de zidovudina e efavirenz
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Data
2009-10-01
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Universidade Federal de Goiás
Resumo
The therapeutic drug monitoring is an important tool to predict the
therapeutic efficacy and possible toxicity of antirretroviral agents used to treat
infection by human immunodeficiency virus (HIV). The goals of this study was to
develop and validate a method of analysis and extraction to quantify the
antirretrovirals efavirenz and zidovudine in human plasma, to know the clinical
profile and quantify the drugs efavirenz and zidovudine in plasma of volunteers
using treatment regimen efavirenz / lamivudine / zidovudine. Blood samples
were collected approximately 8 h after administration of AZT and 20 h of the
EFZ, were obtained from adult volunteers HIV positive in clinic monitoring at
Hospital das Clínicas, Universidade Federal de Goiás and therapeutic protocol
EFZ 600 mg once daily and lamivudine 150 mg + zidovudine 300 mg twice a
day. After solid phase extraction using cartridge Strata 18E C 500mg, 3 mL
capacity, the drugs and internal standard (5 - (4-methylphenyl)-5-fenilidantoína -
MPPH) were separated by reversed-phase column ACE 5 C18 100 x 4 6 mm.
The mobile phase consisted of acetonitrile and diethanolamine in water (pH 7.8),
flow rate 0.5 to 2.0 mL/min and detection at wavelength of 249 nm (EFZ), 267
nm (AZT) and 240 nm (MPPH). The method showed linearity at concentration
range of 50 to 5000 ng/mL for both drugs, with recovery of 84 and 92% for EFZ
and AZT, respectively. The limit of quantification (50 ng/mL), precision
(coefficient of variation <15%) and accuracy (relative errors <15%) are in
accordance with requirements of ANVISA. To evaluate the applicability of the
method, we analyzed plasma of volunteers (n = 12) with mean plasma
concentration of 1960 ± 766 ng/ mL to EFZ and 62.12 ± 16.21 ng/mL to AZT.
The method agree analytical parameters suitable for use in therapeutic
monitoring of patients using AZT and EFZ
Descrição
Citação
SOARES, Amanda Queiroz. Development and validation of methodology for HPLC-PDA for therapeutic monitoring of plasma levels of zidovudine and efavirenz. 2009. 123 f. Dissertação (Mestrado em Ciências da Saúde - Medicina) - Universidade Federal de Goiás, Goiânia, 2009.