Análise Morfométrica de Staphylococcus aureus Meticilina Resistentes Cultivados em Diferentes Concentrações de Cloreto de Sódio e Oxacilina
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2010-04-15
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Universidade Federal de Goiás
Resumo
Methicillin-resistant Staphylococcus aureus (MRSA) has been one of the
most prevalent microorganisms that cause hospitals infections worldwide.
Several studies show that this microorganism is often found colonizing health
professionals. MRSA can cause skin infections from the severe pneumonia,
with high resistance to different antimicrobial agents. This study aimed to
evaluate morphometric changes in MRSA isolated from the saliva of health
professionals, through the use of different concentrations of oxacillin and
sodium chloride. The identification of morphological changes was assessed by
growing the isolates in the following concentrations of Sodium Chloride and
oxacillin: 2μg, 4μg and 6μg and 2%, 4%, 6% and 7.5% and using the means of
computerized morphometry. This technique was tested by microscopic
computed by employing the software Image J 1.38 (HIH USA). Statistical
analysis was performed using the Sigma Stat, version 2.03, and the differences
between the groups were compared using the nonparametric Kruskal-Wallis (p
<0.05). The 20 MRSA analyzed showed no alterations. The present study
showed that Sodium Chloride and oxacilin at concentrations did not alter the
development and morphology of MRSA.
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OLIVEIRA, Ana Cláudia Alves de. Morphometric Analysis of Methicillin Resistant Staphylococcus aureus
Grow in Different Concentrations of Sodium Chloride and Oxacilin. 2010. 101 f. Dissertação (Mestrado em Medicina) - Universidade Federal de Goiás, Goiânia, 2010.