Fenotipagem de bactérias isoladas em hemoculturas de pacientes admitidos na unidade clínica de terapia intensiva de um hospital escola
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2006-03-31
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Universidade Federal de Goiás
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Introduction: In hospitals the bloodstream infections represent a serious complication in critical patients The detection of bacteria in blood cultures is considered an important diagnosis instrument This study aimed to detect and characterize bacteria from blood of clinical intensive care unit admitted patients of a teaching hospital in Goiânia-Goiás in the period of April/2003 to April/2004 Methods: The blood samples were seeded in brean heart infusion broth (Bacto - BD Diagnostic Systems/ USA) incubated until 7 days at 37oC and subcultived on chocolate agar to isolated microorganisms The isolated bacteria were identified by biochemical / enzymatic test and automation technique The identification and antimicrobial susceptibility profile were performed by MicroScan ® system. Results: A total of 304 blood cultures of 195 patients were evaluated Forty-nine (16.1%) bloods cultures were positive The Gram-positive cocci represented 47.7% of isolated and Gram-negative rods 50.0% One (2.3%) coryneform bacilli were isolated The most prevalent specie was Pseudomonas stutzeri (18.2%) The Gram-positive cocci and enterobacterias showed higher resistance rate against ampicillin; 66.7% of staphylococci were oxacillin resistant and 77.8% of pseudomonas were resistant aztreonam Vancomycin and linezolid were the most active agents for Gram-positive cocci and the carbapenems for Gram-negative rods Conclusion: The frequency of positive blood cultures was 16.1% with a similar distribution of Gram-positive and Gram-negative bacteria that showed high resistance rate against antimicrobial agents Due to the impact of bloodstream infection in the hospital context others studies are necessary to support measure of prevention and control
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LEÃO, Lara Stefânia Netto de Oliveira. Fenotipagem de bactérias isoladas em hemoculturas de pacientes admitidos na unidade clínica de terapia intensiva de um hospital escola. 2006. 75 f. Dissertação (Mestrado em Medicina) - Universidade Federal de Goiás, Goiânia, 2006.