Caracterização Genotípica do HIV-1 em Pacientes dos Estados do Tocantins e Mato Grosso
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Data
2008-03-12
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Universidade Federal de Goiás
Resumo
Introduction: There are limited HIV-1
genetic diversity studies performed in Central West and North Brazil subjects.
Objectives: To characterize the HIV-1 genetic diversity in the gag region of patients from the States of Tocantins (TO) and Mato Grosso (MT).
Material and Methods: 130 patients infected with HIV-1 were recruited from 2003 to 2006, at the regional Public Health Central Laboratories (LACEN) from TO (n=70) e MT (n=60). The HIV-1 genetic diversity in the gag region was investigated by heteroduplex mobility analysis HMA (HMA gag kits/AIDS Reagent Program/ NIH/ USA). For the gag region the Nested-PCR was performed employing the primers H1G777/ H1P02 and H1gag1584/ g17. HIV-1 subtypes references were amplified using the primers of Nested-PCR second round. HMA gag consists in the migration analysis of the hybrid formed by HIV-1 subtypes references and patient samples after polyacrylamide gel electrophoresis (5%).
Results: Males were 60% of the study population in TO and 48,3% in MT. The median age was 34 years (21 l71 years) in TO and 35.5 years (4 64 years) in MT. The risk exposure categories of this study group were: sexual exposure (TO: 72.9%, MT: 93.3%), parenteral risk (TO: 4.3%, MT: 3.4%) and vertical case (MT: 1.4%). The HIV-1 subtypes identified by HMA gag were: B (TO: 82.8%, MT: 50.0%), F (TO: 7.8%, MT: 5.6%), C (TO: 4.7%, MT: 11.1%), B/D (TO: 4.7%, MT: 31.5%) and B/F (MT: 1.8%). Subtype B was predominant among men, women and all risk exposure categories.
Conclusions: The genetic diversity analysis of HIV-1 isolates from TO and MT pointed subtype B as the prevalent. The most prevalent second form in TO was subtype F and in MT was subtype C. These results contribute to the genetic molecular mapping of the HIV-1 gag region in the Central West and North Brazil.
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Citação
VIEGAS, ângela Alves. Molecular Screening of HIV-1 Genetic Diversity Among Patients from Tocantins and Mato Grosso. 2008. 100 f. Dissertação (Mestrado em Medicina) - Universidade Federal de Goiás, Goiânia, 2008.