Obtenção e Caracterização do Extrato Seco Padronizado da Rosmarinus Officinalis L. (Lamiaceae)
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Data
2011-02-24
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Universidade Federal de Goiás
Resumo
In this work, standardized spray-dried extracts of Rosmarinus officinalis L.(Lamiaceae), were obtained. The work started with the obtainment and characterization of the
R. officinalis dried and powder leaves. A hydroalcoholic extract was obtained by percolation using as solvent ethanol at 80 % (v/v). The drying experiments followed a Box-Behnken (33) factorial design in order to obtain dry powders with optimized properties. In this way, the influence of several in-process parameters e. g. extract feed rate, drying air inlet temperature and spray nozzle airflow rate, on the dried extracts properties were investigated. The herbal
raw material as well as both hydroalcoholic and dried extracts were analyzed by several physic-chemical and physical techniques. Additionally, the in vitro antioxidant activity from
both hydroalcoholic and dried extracts were assessed by DPPH free radical scavenging test. Rosmarinic acid, the major component on the specie and one of the main compounds
responsible for these pharmacological activities, was used as chemical marker in High Performance Liquid Chromatography (HPLC) coupled to photodiode array detection (PDA)
quantification assays in all steps of this work. The analytical method was validated following Agência Nacional de Vigilância Sanitária (ANVISA Brazilian National Health Surveillance
Agency) guidelines. This method proved to be simple, rapid, selective, linear, sensible, precise, accurate a robust. On the herbal raw material characterization, it were observed a
swelling índex of 2.33 ± 0.11, a mean powder size of 437.94 ± 7.00 μm, as well as moisture, total ashes, acid insoluble ashes, total polyphenols, total tannins, total flavonoids and
rosmarinic contents of 9.1 ± 0.23, 7.32 ± 0.045, 0.378 ± 0.016, 3.81 ± 0.043, 0.49 ± 0.01, 1.73 ± 0.07 and 1.05 ± 0.03% (w/w), respectively. The concentrated hydroalcoholic extract presented density of 0.964 ± 0.002 g/mL, solids content of 9.66 ± 0.07%, pH 5.106 ± 0.005, alcoholic content of 38.2 ± 0.53% and a viscosity of 5.2 ± 0.09 mPas. The levels of total polyphenols, total tannins, total flavonoids and rosmarinic contents were of 30.19 ± 0.24, 9.13 ± 0.01, 8.78 ± 0.1 and 10.68 ± 0.43% (w/w), respectively. Also, in the antioxidant assessment, the extract presented an inhibitory concentration (IC50) of 17.29 μg/mL. Results showed
drying yields ranging from 17.1 to 74.96%. Also, all dried products showed moisture contents and water activities below 5% (w/w) and 0.5, respectively. Although dry products having lost some of their polyphenols they still presented antioxidant activities (IC50) ranging from 17.58
to 24.83 μg/mL. However, most recovered products presented inadequate flowability and compressibility. In general, the particles showed spherical shape and various sizes. Analysis
of variance (ANOVAs) proved that the factors studied and some of their interactions significantly affected most of the investigated responses. Moreover, the best condition for
obtaining dry extracts of rosemary with stability evidence as well as adequate physicochemical and functional properties is the one performed with a high extract feed rate, an
intermediate drying air inlet temperature and a low spray nozzle air flow rate. Accordingly, spray drying technique may be an attractive and promising alternative to develop intermediate phytopharmaceutical products of rosemary.
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Palavras-chave
ácido rosmarínico , atividade antioxidante , fitoterápicos , Rosmarinus officinalis L. , spray drying , tecnologia de pós. , antioxidant activity , phytopharmaceuticals , powder technology , rosmarinic acid , Rosmarinus officinalis L. , spray drying. , 1.Ácido rosmarínico 2. Atividade antioxidante 3. Fitoterápicos 4. Rosmarinus officinalis L (Alecrim)v5. Spray drying 6. Tecnologia de pós
Citação
COUTO, Renê Oliveira do. Obatainment and Characterization of Standardized dry Extracts Form Rosmarinus Officinalis L.(Lamiaceae). 2011. 70 f. Dissertação (Mestrado em Ciências da Saúde - Farmácia) - Universidade Federal de Goiás, Goiânia, 2011.