Atividade antitumoral e antiangiogênica de Synadenium umbellatum Pax in vitro e in vivo
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2008-03-11
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Universidade Federal de Goiás
Resumo
Synadenium umbellatum Pax is a vegetable belonging to Euphorbiaceae, popularly
known as cola-nota , milagrosa , and cancerola , is empirically used as a natural
remedy for fighting cancer. In this study we assessed the antitumoral and
antiangiogenic activity in vitro and in vivo of ethanolic crude extract and hexanic and
chloroformic fractions of S. umbellatum. Citotoxicity assessment tests in vitro were
carried out through MTT reduction and Trypan blue exclusion methods, by using
Ehrlich ascitic tumor cells and K-562 lineage. Antitumoral and antiangiogenic activity
in vivo essays were carried out through Ehrlich ascitic tumor experimental model in
Swiss mice. Ethanolic crude extract was tested in 5, 10 and 25 mg/kg/day doses,
whereas chloroformic fraction was tested in 1.2; 2.3 and 5.8 mg/kg/day doses, and
hexanic fraction in 0.4; 0.8; and 1.9 mg/kg/day doses, for 10 days. The results
showed concentration-dependent citotoxic activity, presenting through MTT method
the inhibitory concentrations of 50% (Cl 50) 0.4, 0.1, and 0.08 mg/ml for ethanolic
crude extract, chloroformic and hexanic fractions, respectively. And in the Trypan
blue exclusion test, cell inhibition was 75%, 68%, and 80%, and 95%, 58%, and 64%
for Ehrlich ascitic tumor cells and K-562 lineage, respectively. Ethanolic crude extract
in the 25mg/kg/day dose increased the animals survival rate, parallely with the
reduction of the amount of tumoral cells, beyond present activity on the tumor
angiogenesis, and reduce vascular endothelial growth factor levels. Chloroformic and
hexanic fractions, in in vivo essays, did not present significant outcomes. Thus, can
conclude in the condition this study, that S. umbellatum shows antitumoral and
antiangiogenic activity
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NOGUEIRA, Iara Antonia Lustosa. Synadenium umbellatum, agentes antineoplásicos, neovascularização, citotoxidade. 2008. 140 f. Dissertação (Mestrado em Ciências da Saúde - Farmácia) - Universidade Federal de Goiás, goiânia, 2008.