DESENVOLVIMENTO E VALIDAÇÃO DE MÉTODO ANALÍTICO DE CROMATOGRAFIA LIQUIDA DE ALTA EFICIÊNCIA E ENSAIO DE DISSOLUÇÃO PARA AVALIAÇÃO DA QUALIDADE DE FITOTERÁPICOS CONTENDO Paullinia cupana Kunth
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Data
2009-07-30
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Universidade Federal de Goiás
Resumo
The Herbal medicines are obtained by using only raw vegetable active.
Guarana (Paullinia cupana Kunth.) is used as an invigoranting and challenging due
to the presence in the seeds of methylxanthines, especially caffeine and theobromine
and theophylline lesser amount, and tannins. The test is an dissolution of the tests
used in assessing the quality of these medicines and throught this you can evalueted
the release of the active principle from a solid form in an environment that simulates
the location of its absorption. This study aimed to evaluete the behavior of dissolution
of capsules and tablets containinf guarana. This study investigated initially,
spectrophotometric and chromatographic methods for quantification of tannins and
methylxanthines in pharmaceutical forms and the means of dissolution. The results
obtained by spectophotometry showed lack of specificity of this method under our
experimental conditions for quantification of methylxanthines. In the analysis of
tannins, the procedure is not feasible for an routine laboratory to be long and
larorious. The method by High Performance Liquid Chromatography allowed the
quantification of the five markers simultaneously, with precision, accuracy, selectivity
and robustness. The dissolution test using apparatus as man, 37 ºC ± 0,5 ºC, speed
of 75 rpm in 900 mL of 0.1 M HCl medium pH 1,2 provided the best results was
validated and used in assessing the behaviour of dissolution of the capsules and
tablets based on guarana. It was found that all markers were quantified by the
method are dissolved between 75% and 80% in time of 10 minutes. An analysis of
the capsules did not break in any of the conditions employed. For the tablet was also
observed in non-compliance, non-identification of catechin and epicatechin in the
finished product, worrying factor, as the phenolic compounds are markers that should
be present in the guarana powder. Considering the increasing use of plants is a clear
need for definition of quality standards to ensure its therapeutic action and
accordingly the study of dissolution is an essential tool in ensuring the quality of
plants
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Citação
SOUSA, Sandra Alves de. DEVELOPMENT AND VALIDATION OF ANALYTICAL METHODS
LIQUID CHROMATOGRAPHY AND HIGH PERFORMANCE TEST
SOLUTION FOR QUALITY ASSESSMENT CONTAINING HERBAL cupana Kunth Paullinia. 2009. 112 f. Dissertação (Mestrado em Ciências da Saúde - Farmácia) - Universidade Federal de Goiás, Goiânia, 2009.