PRODUÇÃO DE LACASE E BIOCONVERSÃO DE FLAVONÓIDES POR Pycnoporus sanguineus.
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Data
2009-06-30
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Universidade Federal de Goiás
Resumo
The bioconversion is an area of the biotechnology that has increased in an
expansive way, and it includes enzymatic reactions by microorganisms. These
microorganisms that present enzymatic systems similar to those present in
mammalian systems, it s the cytochrome P450 (CYP450). That s why the
biotransformation an important alternative constitute as models for drug
metabolism study. The Pycnoporus sanguineus is a filamentous fungi,
basidiomycete of the Polyporaceae family and laccase producer (EC 1.10.3.2), an
oxidoreductase enzyme. It was evaluated the influence of flavonoids naringin,
naringenin, and quercetin in the growth of P. sanguineus, production of laccase in
differents culture media. It was performed various conditions of reaction, such as
temperature, agitation and the time of the flavonoids addition. The bioconversion
processes were carried out for 24 up to 96 hours and monitored by TLC and
HPLC to confirm the existence of potential metabolites. It was used purified
laccase of the Pycnoporus sanguineus to evalute the participation of laccase in
metabolites of naringin production. Pycnoporus sanguineus developed in
differents culture media tested. Naringenin and naringin presented the capability
to induce the laccase production by P. sanguineus, whereas quercetin and rutin
inhibited the laccase production. The incubation using PDSM did not producted
laccase. It was detected several of metabolites, whereas the incubations using
quercetin acquired fourteen differents metabolites and the incubations using
naringenin producted nine metabolites and naringin producted eight metabolites.
In biocatalytic assay under 28ºC and 150rpm using naringin it was not detected
the presence of metabolites
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BATISTA, Francislene Lavôr. PRODUCTION lacquers and bioconversion
FLAVONOIDS BY Pycnoporus sanguineus. 2009. 82 f. Dissertação (Mestrado em Ciências da Saúde - Farmácia) - Universidade Federal de Goiás, Goiânia, 2009.