Pesquisa de anticorpos IgG séricos anti-lipoproteínas de mycoplasma fermentans e mycoplasma hominis ou anti-mam (superantigeno de mycoplasma arthritidis) em pacientes com artrite reumatoide ou lupus eritematoso sistemico
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Data
2008
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Editor
Universidade Federal de Goiás
Resumo
Rheumatoid Arthritis (RA) and Systemic Lupus Erythematosus (SLE) are
autoimmune diseases of unknown etiology. Some species of mycoplasmas
cause arthritis in animals and humans, and their lipid-associated membrane
proteins (LAMPs) and Mycoplasma arthritidis mitogen (MAM superantigen) are
potent stimulators of the immune system. Thus, it has been proposed that
mycoplasma can be involved in autoimmune-disease etiology. The objective of
the present work was to detect antibodies to MAM and LAMPs of M. hominis and
M. fermentans in the patient sera, and to characterize the profile of IgG
antibodies reactivity with LAMPs in order to identify the major immunogenic
mycoplasmal lipoproteins that could be involved in the etiopathogenesis of these
autoimmune diseases. Serum samples were obtained from peripheral blood of
female patients at the same age of healthy controls. Recombinant MAM (from M.
arthrititidis), LAMPs of M. hominis PG21 and M. fermentans PG18 were used in
Western blotting assays. Antibodies to MAM were detected in the patient and
control sera (RA: 27.5% vs 18.8%; SLE: 21.7% vs 20.0%). At least 23 LAMPs
were found in the preparations of M. hominis PG21 and of M. fermentans PG18
with molecular masses between 20 and 192 KDa. The sera of RA patients
recognized a larger number of LAMPs of M. hominis PG21 and M. fermentans
PG18 than the control sera (RA: 11 ± 4 vs controls: 7 ± 3, n = 35; p < 0,05). Most
of the sera of RA patients presented strong reactivity with LAMPs of M. hominis
PG21 (RA: 65.7% vs controls: 20%, p < 0.05). LAMPs of M. hominis PG21 with
molecular masses < 49 and ? 20 KDa and LAMPs of M. fermentans PG18 < 102
and ? 58 were mainly recognized by IgG antibodies of RA patients. When
comparing sera from SLE patients and controls there was detected no significant
differences between the profiles of IgG reactivity. Therefore, M. hominis PG21
LAMPs (< 49 and ? 20 KDa) and M. fermentans PG18 LAMPs (< 102 and ? 58
KDa) are high immunogenic mycoplasmal antigens that can induce antibody
cross reactivity with self antigen, contributing with the RA pathogenesis.
Descrição
Palavras-chave
rMAM , Micoplasmas arthritidis , Lipoproteínas , Anticorpos IgG , Lúpus eritematoso sistêmico , Mycoplasma homini , Mycoplasma fermentans , Artrite reumatóide , rMAM , Mycoplasma arthritidis , Mycoplasma hominis , Lipoproteins , IgG antibodies , Rheumatoid arthritis , Systemic lupus erythematosus , Mycoplasma fermentans
Citação
Rocha Sobrinho, Hermínio Maurício da. Pesquisa de anticorpos IgG séricos anti-lipoproteínas de mycoplasma fermentans e mycoplasma hominis ou anti-mam (superantigeno de mycoplasma arthritidis) em pacientes com artrite reumatoide ou lupus eritematoso sistemico. 2008. 79 f. Dissertação (Mestrado em Medicina Tropical e Saúde Pública) - Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Goiânia. 2008.