Lesões de isquemia arteriovenosa e reperfusão em jejuno de equinos: imunoistoquímica de proteínas de junção e histopatologia
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2007-12-31
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Universidade Federal de Goiás
Resumo
To evaluate whether complete arteriovenous ischemia followed by
reperfusion of mesenteric blood flow aggravates lesions involving small
intestine of horses, β--catenin and occludin expressions were measured in
jejune epithelia of seven equines, without definite breed. The animals had
been submitted to aseptic ventral midline celiotomy and five jejune
segments collected corresponding to a baseline segment, a five minutes
segment subjected to ischemia, and three segments collected after being
subjected to five, sixty and one hundred and twenty minutes of tecidual
reperfusion, respectively. Experimentally induced lesions created by total
arteriovenous occlusion followed by reperfusion were similar to naturally
acquired lesions, as much in the period of ischemia as in the aggravation of
the injuries observed during reperfusion. Changes that occur when ischemic
intestine is reperfused are progressive, and include mucosal, submucosal,
and serosal edema; polymorphonuclear and mononuclear cell infiltrates;
and alterations in vascular patterns. Imunohistochemistry analyses have
shown strong staining baseline segments for β-catenin in all animals and its
progressive lack of staining during ischemia and reperfusion treatments.
That indicates progressive lack of intercellular adherence. Similar results of
specific decreases of total expression of Occludin, a tight junction protein, were
observed. This protein is involved in regulating the movement of solutes in the
paracellular pathway and also plays an important role in the maintenance of cell
polarity. The reduction of expressions of both intercellular proteins measured
directly demonstrates β-catenin injury due to progression of edema during
ischemia and its aggravation during reperfusion and also shows an increase
of the permeability of paracelular pathways and its related consequences to degradation of Ocludina.
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NASCIMENTO, Aline Machado Rapello do. Lesões de isquemia arteriovenosa e reperfusão em jejuno de equinos: imunoistoquímica de proteínas de junção e histopatologia. 2007. 105 f. Dissertação (Mestrado em Ciências Agrárias) - Universidade Federal de Goiás, Goiânia, 2007.