Redução do Acefato utilizando lacases produzidas por Trametes villosa e Pycnoporus sanguineus com Trichodermas isolados do Cerrado
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Data
2011-08-23
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Universidade Federal de Goiás
Resumo
The organophosphate insecticides have been widely used for agricultural purposes. In the environment, organophosphates have been found in various environmental matrices, resulting in a growing environmental concern, among these compounds
stands out Acephate, organophosphate and was registered by ANVISA reassessed in 2009, due to their toxicological and carcinogenic potential. The development of this work sought to select strains of Trichoderma harzianum capable of stimulating enzyme production by Trametes villosa and Pycnoporus sanguineus to promote the reduction of toxicity of Acephate. First there was the selection of strains of T.
harzianum then became mixed cropping of selected strains of T. harzianum with Trametes villosa and T. harzianum with Pycnoporus sanguineus to determine the potential production of enzymes, and further tests were carried out with
concentrations of 10% and 50% Acephate then held the toxicity test to assess the reduction of toxicity of Acephate. The results obtained in the experiments show that linhanges T. harzianum are good producers of lignin peroxidase (45 U.mL-1) and manganese peroxidase (23 Uml-1), the strains of T. harzianum were able to increase the production of Laccase in culture with T. villosa (20.57 Uml-1) and assessed that the fungi produced more enzymes in the presence of 50% Acephate. In toxicity tests, the samples with 10% Acephate addressed by the fungi indicated that compared to control, the association of P. sanguineus and Trichoderma T47
increased by 94% of cholinesterase, indicating a decrease in its toxicity, the association of P. sanguineus and Trichoderma harzianum ALL42 increase of 93% and the association between T. villosa and T. harzianum T39, an increase of 100%
of cholinesterase, indicating that fungi were able to reduce the toxicity of Acephate.
Descrição
Palavras-chave
Acefato , Biorremediação , Lacase , Trichoderma harzianum , Trametes villosa , Pycnoporus saguineus , Acephate, Bioremediation , Laccase , Trichoderma harzianum , Trametes villosa , Pycnoporus saguineus , 1. Acefato; 2. Biorremediaç; 3. Lacase;
4. Trichoderma harz; 5. Trametes villosa;
6. Pycnoporus sanguineus
Citação
SILVA, Carolina Braz. Reduction of the laccases produced by using acephate Trametes villosa and Pycnoporus sanguineus Trichoderma with isolated from Savanna. 2011. 119 f. Dissertação (Mestrado em Engenharias) - Universidade Federal de Goiás, Goiânia, 2011.