Ocorrência de Prototheca zopfii em propriedades leiteiras do estado de Goiás e avaliação do limite de sensibilidade para o seu isolamento em leite
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2008-08-05
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Universidade Federal de Goiás
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Mastitis is the most frequent disease in dairy cows, it causes great economic losses in herds and dairy industries and represents a hazard for public health. Among the etiologic agents identified, there is a predominance of bacteria species. However, several other groups of microorganisms may be present, one of those is a unicellular alga of the Prototheca genus, the Prototheca zopfii, an environmental agent of bovine mastitis. The infected cows usually have granulomatous changes in mammary tissue. Moreover microorganisms do not respond to routine therapy, resulting in the elimination of the infected animals. The main purposes of this study was to determine the occurrence of Prototheca zopfii on bulk tank milk in the state of Goiás and identify the limit of detection for Prototheca zopfii by two different approaches. We also wanted to know the samples pre-incubated with small concentration of Protoheca zopfii permits us to obtain positive growth in microbiological culture. We analyzed 473 samples from farms with high somatic cell count patterns but we did not detect Protoheca zopfii in any of those samples. The limit of sensitivity of the Prototheca zopfii was analyzed, in the approach of isolation the limit of detection was between 130 to 3,400 UFC/mL. In the approach of tally the limit was between 25 to 300 UFC/mL. It wasn t observed significant difference (p>0,05) between the approaches analyzed. However, significant statistical difference (p<0,05) occurred between the five types analyzed. After the pre-incubation at 37ºC/24h, the limit of sensibility was analyzed by the approach isolation, the limit was 30 UFC/mL, what means that the pre-incubation is so important to decrease the possibility of occurrence of false negative
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TUNDELA, Eveline Silva Xavier. Occurrence of Prototheca zopfii on dairy farms in the state of Goiás and evaluation of the limit of sensitivity for its isolation
in milk. 2008. 51 f. Dissertação (Mestrado em Ciências Agrárias - Veterinaria) - Universidade Federal de Goiás, Goiânia, 2008.