Identificação de herpesvírus bovino em amostras de cérebro
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2011-02-24
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Universidade Federal de Goiás
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Herpetic meningoencephalitis is an infection of the central nervous system caused by bovine herpesvirus 5 (BoHV-5) despite BoHV-1 also being associated with this pathology. The course of the disease is usually fatal. Meningoencephalitis has caused significant economic burden to the Brazilian cattle industry due to its high mortality rate, especially in young animals. Because of cross-reactivity and the absence of an effective serological test that differentiates types 1 and 5 of herpesvirus, the actual prevalence of infection with both viruses is unknown. It is estimated that some animals, supposedly infected with type 1, may be seropositive for type 5. For a better understanding of the molecular epidemiology of this virus in Goiás, 110 brain samples of young cattle, that died with neurological signs, referred to the reference laboratory of the State of Goiás for rabies diagnosis in 2008, were analyzed by multiplex PCR to amplify the glycoprotein C (gC) region of the BoHV-1 and -5 DNA. Of the 110 samples, 53.6% were positive for bovine herpesvirus. Of these, 22.0% were positive for BoHV-1, whereas 52.5% were positive for BoHV-5. Furthermore, 13.6% of samples showed co-infection for types 1 and 5. Among these, one sample came from a buffalo. Positivity for both bovine herpesvirus and rabies virus was observed in 53.3% of samples. This study showed that BoHV-1 and -5 are circulating among cattle in the State of Goiás and that herpesvirus type 1 can also be encephalitogenic. Although there was no evidence of viral replication, after inoculation of all 20 brain samples in cell culture, the possibility of viruses being associated with neurological disease cannot be ruled out. This was the first study to demonstrate the presence of BoHV-1 and BoHV-5 in samples obtained from herds in Goiás.
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SILVA, Duanne Alves da. Identification of bovine herpesvirus in brain samples. 2011. 64 f. Dissertação (Mestrado em Ciências Agrárias - Veterinaria) - Universidade Federal de Goiás, Goiânia, 2011.