PERFIS DE ERIC-PCR DE Escherichia coli E E. coli O157:H7 EM MEIAS-CARCAÇAS BOVINAS
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Data
2008-08-27
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Universidade Federal de Goiás
Resumo
There are different sorotypes of Escherichia coli responsable for enteric
disfunctions, and in the most of time those are related with meat consume or
contaminated food that had not been cooked with an efficient thermal treatment
before been ingested. In Brazil there aren t inform data of possible outbreaks
caused by E. coli O157:H7, but this pathogen has been frequently isolated from
cattle s feces, and it was recently isolated from bovine carcass at two
slaughterhouses in Goiás. The present study has the objective to characterize
by ERIC-PCR E. coli and E. coli O157:H7 detected at bovine carcass surfaces
and check the power of this methodology to identify different isolates of E. coli
and E. coli O157:H7. ERIC-PCR was used to characterize 111 samples, and it
was obtained 32 fingerprints separated in 90 isolates of E. coli and eight
fingerprints separated in 16 isolates of E. coli O157:H7. From the total of 111
samples, two isolates of E. coli and five of E. coli O157:H7 were non-tipables.
The fingerprints varies from one to 18 bands. The discrimination between the
samples was high, showing the big power of ERIC-PCR to discriminate isolates
from one specie. The discriminatory index between E. coli and E. coli O157:H7
obtained was 0,96.
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Citação
COSTA, Joice Vinhal. PROFILES ERIC-PCR of Escherichia coli and E. coli O157: H7 IN
HALF-CARCASSES BOVINE. 2008. 91 f. Dissertação (Mestrado em Ciências Agrárias - Veterinaria) - Universidade Federal de Goiás, Goiânia, 2008.