Nova abordagem para análise de neurotransmissores em tecidos cerebrais por DESI-imaging
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2021-02-08
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Universidade Federal de Goiás
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Mass spectrometry imaging using desorption electrospray ionization (DESI-imaging) is a method with the potential to be applied to investigate the distribution of neurotransmitters (biogenic amines and amino acids) in brain tissues. Despite the low ionization efficiency of neurotransmitters in direct tissue analysis by DESI, due to signal suppression by lipids, neurotransmitters can be analyzed using derivatization protocols that insert a known compound into their structures through a chemical reaction. In this sense, this work aims to apply chemical derivatization protocols in brain tissues to be analyzed by DESI-imaging. The derivatization used the 2,4,6-triphenylpyrylium tetrafluoroborate (TPP+) reagent and was first investigated in a solvent in order to ensure the detection of the expected products. Subsequently, the brain tissue surface was subjected to the derivatization reaction through the application of TPP+ by spraying. Then, the tissue was analyzed by DESI, and the ions derived from glycine (Gly), alanine (Ala), dopamine (DOPA), and gamma-aminobutyric acid (GABA) were detected. In addition, the DESI-imaging analysis demonstrated distinct spatial distributions for these derivatized neurotransmitters. This protocol was applied in the study of neurotransmitters in mice with Parkinson's, and it was possible to verify differences in the intensities and anatomical distribution for these derivatives in control and diseased (affected by Parkinson's disease model) tissues. Thus, the derivatization/DESI-imaging protocol described in this work emerges as a potential tool for analyzing Parkinson's Disease biomarkers in mouse brain tissues.
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MACIEL , L. Í. L. Nova abordagem para análise de neurotransmissores em tecidos cerebrais por DESI-imaging. 2021. 75 f. Dissertação (Mestrado em Química) - Universidade Federal de Goiás, Goiânia, 2021.