Desenvolvimento de dispositivos microfluídicos em poliéster-toner para diagnósticos clínicos com detecção colorimétrica
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2013-08-28
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Universidade Federal de Goiás
Resumo
This report describes the fabrication of a minimally instrumented polyestertoner
device (PTD) to perform by the first time, clinical diagnostics with
colorimetric detection and microfluidic transport by capillary action. A
conventional digital camera, a cellphone camera, an optical microscope, and
a scanner mode from a multifunction printer were evaluated for using as
colorimetric detector. In order to study the analytical performance it was used
a platform containing nine detection zones directly printed on a polyester film.
It has been observed that all electronic devices studied could be applied as
colorimetric detector. However, the scanner exhibited the best performance
on the control of two problems often observed in the images acquisition,
which are related to the ambient light and focus. Due to the best results, the
analytical feasibility of the scanner was evaluated for the determination of
Fe2+ in drugs samples by using the 1,10-phenanthroline method. The data
were compared to results achieved by a reference spectrophotometric
method. Based on a statistical analysis, it was not been found significance
differences (P=0,05). Afterwards, the scanner was chosen to monitor the
colorimetric response of clinical assays regarded to the detection of glucose,
total protein, cholesterol, and triglycerides on a PTD. The proposed device
exhibited good inter and intra-device reproducibility (relative standard
deviation values lower than 6%). A semi-quantitative analysis of glucose,
total protein, and cholesterol in an artificial serum sample provided results
similar to the data provide by the supplier. The shelf life of PTDs was
evaluated in a period of five days, using the glucose assay at three different
temperature: 10 ºC, 25 ºC e 40 ºC. The shelf life was estimated to be c.a.
three days, taking into account that in this period there was a lost of 50% in
the mean color intensity. Lastly, it was observed that the addition of a
disaccharide (trehalose) ensures the enzymatic stability at the different
temperatures during five consecutive days. The cost of a PTD has been
estimated to be ca. R$ 0.40 taking into account the fabrication process.
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SOUZA, Fabrício Ribeiro de. Desenvolvimento de dispositivos microfluídicos em poliéster-toner para diagnósticos clínicos com detecção colorimétrica. 2013. 97 f. Dissertação (Mestrado em Química) - Universidade Federal de Goiás, Goiânia, 2013.