Avaliação do efeito vasorelaxante das folhas de Caryocar brasiliense camb. em aorta torácica de ratos
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2012-07-17
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Universidade Federal de Goiás
Resumo
Caryocar brasiliense Camb. (“pequi”) is a native plant from the Cerrado
region of Brazil that contains several bioactive components. Data from literature has
demonstrated that dietary supplementation with pequi decreased the arterial
pressure of volunteer athletes. In this work, we evaluated the vasorelaxant effect of
the crude hydroalcoholic extract (CHE) of C. brasiliense leaves, and its organic
fractions (hexane (HF), chloroform (CF), ethyl acetate (AEF), butanol (BF)), in the rat
thoracic aorta. We found that CHE completely relaxed, in a concentration-dependent
manner, rat aortic rings precontracted with phenylephrine, while butanolic fraction
(BF) produced an effect similar to that of the CHE. Induced aortic relaxation BF by
was abolished by endothelium removal, by incubation with the nitric oxide synthase
inhibitor (L-NAME), and the soluble guanylate cyclase inhibitor (ODQ). However,
incubation with either atropine (a muscarinic receptor antagonist) or pyrilamine (a
histamine H1-receptor antagonist) had no effect on the BF-induced vasorelaxation.
Moreover, this effect was not inhibited by indomethacin (a cyclooxygenase inhibitor)
and tetraethylammonium (a non-selective K+ channel blocker). The vasorelaxation
induced by BF in endothelium-intact aortic rings precontracted with KCl was reduced
after incubation with L-NAME. Taken together, the results reveal that C. brasiliense
possesses in vitro vasorelaxant effect in rat thoracic aorta and this effect involves
stimulation of the nitric oxide/cyclic GMP pathway.
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OLIVEIRA, Lais Moraes de. Avaliação do efeito vasorelaxante das folhas de Caryocar brasiliense camb. em aorta torácica de ratos. 2012. 73 f. Dissertação (Mestrado em Biologia) - Universidade Federal de Goiás, Goiânia, 2012.