Enxertos de gordura associados a plasma rico em plaquetas em ratas - estudo experimental
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2013-07-25
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Universidade Federal de Goiás
Resumo
Autologous fat grafts used for filling soft tissue defects have been used for
more than a century. Fat is considered an ideal filler because of its low cost,
ease of harvest, abundance in the human body and low immunogenic and
allergic reaction due to its autologous nature, being largely used in aesthetic
and reconstructive plastic surgery. Platelet rich plasma (PRP) is a plasma
fraction, with platelet count above baseline, generally obtained via
centrifugation of blood. PRP theoretically promotes tissue regeneration due
to fact that it concentrates a greater amount of growth factors essential in the
process of tissue regeneration and neovascularization. This paper aims to
examine if the association of fat grafts and PRP improves graft viability in
female rats, through an experimental, randomized and blinded study, which
involved 47 animals. These animals underwent fat graft harvest from their
inguinal fat deposits and fat grafting subcutaneously to their cranial region. In
22 animals the fat graft was mixed with PRP and in 25 the fat was grafted by
itself. After a 100 day period, the animals were sacrificed and the fat grafts
were analyzed using scores from 0 (absent) to 4 (abundant), in optical
microscopy by two independent and blinded pathologists, by means of the
following variables: fat graft cell viability, fat necrosis, tissue inflammation and
fibrosis. Regarding fat graft cell viability, the PRP group scored
moderate/abundant in 63% of the cases and the fat graft only group scored
absent/slight in 72% of the cases (p<0.05). The PRP group presented lower
fat necrosis scores and lower tissue inflammation scores when compared to
the fat graft only group (p<0.05). The presence of tissue fibrosis was rarely
observed in both groups. Tumors (dermoid cysts) within the fat grafts were
observed in 3 animals in which the grafts were mixed with PRP. It is
concluded that PRP improves the viability and integration of fat grafts in rats,
but more studies are needed to fully understand the exact mechanisms that
lead to this improvement and assess the safety of the method for use in
humans.
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BLUMENSCHEIN, Alexandre Roriz. Enxertos de gordura associados a plasma rico em plaquetas em ratas - estudo experimental. 2013. 56 f. Dissertação (Mestrado em Ciências da Saúde) - Universidade Federal de Goiás, Goiânia, 2013.