Reatividade sorológica a proteínas recombinantes do Mycobacterium leprae em diferentes grupos populacionais de distintas regiões endêmicas do Brasil
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2013-02-27
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Universidade Federal de Goiás
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The development of laboratory tests applicable for the diagnosis/classification of the different clinical forms of leprosy is considered a research priority. The completion of Mycobacterium leprae genome together with new gene cloning/expression techniques and new bioinformatic tools have promoted the production and availability of new M. leprae recombinant proteins for immunologic assessment. Goal: This study assessed the serologic reactivity to M. leprae recombinant proteins among leprosy patients and controls from two hiperendemic regions in Brazil: “Rondonópolis/MT” and “Vila do Prata/Igarapé-Açú/PA” (former leprosy colony). Material and Methods: IgG antibodies to M. leprae recombinant proteins (92f, 46f, LID-1, ML0405 e ML1213; 2 g/ml) and IgM antibodies for the PGL-I synthetic trissacaride (NT-P-BSA; 0.01 g/ml) were detected by ELISA. The following study groups were included (n=847): newly diagnosed untreated leprosy patients (paucibacillary- PB and multibacillary- MB), household contacts of MB patients (HHC), healthy endemic controls (EC) and former MB that concluded leprosy multidrug therapy (MDT) (post MDT). Results: Among participants from Rondonópolis/MT (n=764), the seropositivity of MB patients (n=58) was 59% for 92f, 81% for 46f, 89% for LID-1, 84% for ML0405; 83% were anti-PGL-I positives. Among 10 anti PGL-I negative MB patients, 5 recognized LID-1 e 4 had IgG antibodies to 92f, 46f and ML0405. Among PB leprosy patients (n=93) seropositivity rates to M. leprae recombinant proteins ranged from 5-16%; 8% were anti PGL-I positives. In the HHC (n=192) and in the EC groups (n=282) low reactivity rates were detected ranging from 3-7%. For the post MDT group, positivity rates ranged from 17-45%. Among participants from “Vila do Prata” 3-8% of HHC were seropositives for recombinant proteins; 11% were anti- PGL-I positives. In the post MDT group from “Vila do Prata” (n=47) 33-50% were seropositives for recombinant proteins. Conclusion: High positivity rates to M. leprae- 46f, ML0405 e LID-1 were detected among MB leprosy patients with different genetic/ethnic profiles from distinct geographical regions. These results indicate that the development of a serologic test for leprosy employing both M. leprae recombinant proteins and PGL-I can potentially detect the majority of MB patients from different hiperendemic regions. The use of this diagnostic tool by the public health system can contribute for the early diagnosis and treatment of MB disease which are crucial for the control/elimination of leprosy in Brazil.
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PINTO, Emerith Mayra Hungria. Reatividade sorológica a proteínas recombinantes do Mycobacterium leprae em diferentes grupos populacionais de distintas regiões endêmicas do Brasil. 2013. 104 f. Dissertação (Mestrado em Medicina Tropical e Saúde Pública) - Universidade Federal de Goiás, Goiânia, 2013.