Genotipagem do vírus da hepatite C e avaliação da resposta ao tratamento em Goiânia-Goiás, com ênfase no polimorfismo relacionado ao gene IL28B
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Data
2014-02-28
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Universidade Federal de Goiás
Resumo
Hepatitis C represents a public health problem worldwide. Hepatitis C virus (HCV) is
classified into seven genotypes and many subtypes. Besides their epidemiological
importance, these genotypes have influence on the response to hepatitis C treatment, as
well as other factors related to the virus and its host, such as polymorphisms upstream
of interleukin (IL) 28B locus. Despite the importance of these factors in the response to
treatment of hepatitis C, there are no data regarding the subject in the Midwest Region
of Brazil. The present study aimed to identify the genotypes of HCV among patients
attended at a reference laboratory in Goiânia-GO, and also to assess response to
treatment of patients infected with genotype 1 with pegylated interferon (PEG-IFN) and
ribavirin (RBV), with emphasis on the polymorphism upstream of IL28B gene (rs
12979860). For HCV genotyping, a cross-sectional study was conduct in an anti-HCV
positive patients in a reference laboratory in Goiânia-GO, during in a period of 10 years
(2003 to 2012) (n = 1300). From January/2012 to December/2013 (n = 101), a cohort
was conduct among patients infected with HCV genotype 1 treated with PEG-IFN and
RBV in order to evaluate treatment response. Patients were interviewed and blood
samples collected for detection of viral RNA by polymerase chain reaction (PCR) with
primers complementary to the region 5’ noncoding (NC) of HCV. All HCV RNA
positive samples were genotyped by line probe assay – LiPA, and the samples typed as
1a/1b, 2 and 4 were sequenced in the NS5B region of the viral genome. The analysis of
single nucleotide polymorphism (SNP) rs12979860 C/T was performed by restriction
fragment length polymorphism (RFLP). Of the 1300 samples tested for HCV RNA, 894
were positive. Among these, genotypes 1 (76.3%), 2 (1.9%), 3 (21.6%) and 4 (0.2%)
were found. These data show the predominance of genotype 1, followed by 3 and 2, and
support the results of other studies in Goiânia. In addition, genotype 4 was found in the
studied population. Regarding to SNP rs12979860 C/T, the TC genotype was the most
common (57.4%), followed by CC (23.8%) and TT (18.8%). The rate of sustained
virologic response (SVR) was 28.7%, being higher in individuals with the CC genotype
(54.2%) when compared to those with CT and TT genotypes (22.4% and 15.8%,
respectively). These results indicate that analysis of SNP rs12979860 is an important
predictor of SVR following PEG-IFN and RBV treatment in patients infected with HCV
genotype 1 in our region.
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SILVA, Ágabo Macêdo da Costa e. Genotipagem do vírus da hepatite C e avaliação da resposta ao tratamento em Goiânia-Goiás, com ênfase no polimorfismo relacionado ao gene IL28B. 2014. 118 f. Dissertação (Mestrado em Medicina Tropical e Saúde Pública) - Universidade Federal de Goiás, Goiânia, 2014.