Obtenção de extratos padronizados a partir das cascas de Stryphnodendron adstringens (Mart.) Coville (Fabaceae) e avaliações biológicas in vitro
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2014-03-28
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Universidade Federal de Goiás
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The present work aimed to obtain extracts from the barks of Stryphnodendron
adstringens (Mart) Coville (Fabaceae) and evaluating of biological activities in vitro,
from them. Chapter 1 using pharmacopoeial and not pharmacopoeial methods;
barks, powder and liquid extract of S. adstringens were characterized and then a
HPLC method was developed and validated to idenfication of epigallocatechin gallate
(EGCG) at extracts. In Chapter 2, described the cytotoxic activity of the dry extract of
S. adstringens, obtained by atomization, and the EGCG standard against L929
fibroblast cells by the method of neutral red. EGCG at 200 µg/mL reduced, p ≤ 0.05,
the cell viability of L929 fibroblasts to 47.70%, such as the dried extract of S.
adstringens at 225 mg/mL. Chapter 3 presented the obtainment of dry extracts of S.
adstringens by atomization. The influence of the proportion of adjuvant drying, the
drying air temperature and flow rate of the drying air on total phenols, EGCG,
tannins, total antioxidant activity and efficiency of the drying process were evaluated,
the significant results (p≤0.05) were obtained by ANOVA and represented by
response surface methodology. The increase in the adjuvant drying was not
significantly favorable to efficiency of the drying process. Increasing the temperature
of the drying air had a quadratic behavior negative to total phenols and positive to
total tannins. The increasing of flow air drying showed linear and quadratic behavior.
negative to total antioxidant activity. The efficiency of the drying process was not
influenced by any variable. The dried extracts were analyzed for their content of
volatile, water activity and the morphologies of the particles were observed by
scanning electron microscopy. In Chapter 4 we describe the optimization of
extraction of total phenols, tannins and epigallocatechin gallate powders of barks of
S. adstringens ultrasonic using Box- planning Benhken 33. The use and validation of
the desirability global statistical showed that the proportion of ratio drug solvent
4mg/mL, time extraction 30 min and the 65 % (v/v) hydroalcoholic solution are ideal
to simultaneous extraction of these metabolites. The results indicate that the extracts
were obtained and standardized quality, and have potential to be investigated their
biological and/or insertion activities in herbal formulations
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SOUSA, Jordana Nunes de. Obtenção de extratos padronizados a partir das cascas de Stryphnodendron adstringens (Mart.) Coville (Fabaceae) e avaliações biológicas in vitro. 2014. 138 f. Dissertação (Mestrado em Ciências Farmacêuticas) - Universidade Federal de Goiás, Goiânia, 2014.