Pesquisa de bactérias com determinação do perfil de sensibilidade em vísceras comestíveis de frango de corte, penas e camas de aviários
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2013-11-26
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Universidade Federal de Goiás
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Therefore the study aimed to investigate the presence of these bacteria in edible offal, feathers and poultry litter. Relate the presence of these bacteria in different scores macroscopic changes and determine the sensitivity of E. coli and Staphylococcus antimicrobials of importance in veterinary medicine. For it was collected 13 samples of manure directly in the sheds, abattoir were collected in 12 lots of feathers, plucking directly into 12 lots of heart and liver, the inspection line, totaling 740 samples 740 samples of heart and liver. We established three scores for injuries to the heart and six for the liver. From these samples was carried out the isolation of the bacteria E. coli, coagulase positive Staphylococcus, Pseudomonas spp. and Salmonella sp.. Positive for E. coli and Staphylococcus co were tested for sensitivity to the following antibiotics: ciprofloxacin (5 mg), ceftiofur (25 mg), chloramphenicol (30 mg), erythromycin (15 mg) , tetracycline (30 mg) , trimethoprim-sulfamethoxazole (25 mg) , enrofloxacin (5 mg), oxacillin (1 mg) and gentamycin (10 mg). In addition to those for E. coli was also used colistin (20 mg) and Staphylococcus coagulase positive bacitracin (10 mg). In any sample analyzed was identified Salmonella sp. were found in hearts, 33.1%, 6.6% and 28.2% of E. coli, coagulase positive Staphylococcus and Pseudomonas spp. respectively. In livers 45.4%, 8.0% and 23.1% of E. coli, coagulase positive Staphylococcus and Pseudomonas spp. respectively. To feather the results were 29.2%, 16.7% and 35.8% of E. coli, coagulase positive Staphylococcus and Pseudomonas spp. respectively. In the samples of poultry litter was not isolating Staphylococcus isolates of E. coli and Pseudomonas spp. were 92.3% and 7.7% respectively. In antimicrobial susceptibility testing on samples of E. coli and Staphylococcus coagulase positive bacteria showed the following percentage of antimicrobial susceptibility respectively: ciprofloxacin (36.7% and 58.8%), ceftiofur (8.1% and 64.7%), chloramphenicol (81.0% and 76.5%), erythromycin (2.9% and 41.2%), tetracycline (30.9% and 41.2%), Trimethoprim -sulfamethoxazole (43.8% and 82.3%), enrofloxacin (18.6% and 47.1%), oxacillin (3.8% and 47.1%) and gentamicin (37.1% and 64.7%). For isolates of E. coli tested for sensitivity to colistin 96.7% were sensitive. And for Staphylococcus tested for sensitivity to bacitracin, no specimens were susceptible to this antimicrobial agent.
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CORRÊA, Fernando Augusto Fernandes. Pesquisa de bactérias com determinação do perfil de sensibilidade em vísceras comestíveis de frango de corte, penas e camas de aviários. 2013. 59 f. Dissertação (Mestrado em Ciência Animal), Universidade Federal de Goiás, Goiânia, 2013.