Infecção experimental em suínos por Leptospira interrogans sorogrupos icterohaemorrhagiae e pomona
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Data
2014-05-30
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Universidade Federal de Goiás
Resumo
Swine leptospirosis is a worldwide anthropozoonosis caused by spirochetes of the
species Leptospira interrogans, predominantly by serovars Pomona,
Icterohaemorrhagiae, Taravossi, Canicola Grippotyphosa and Bratislava.
Experimental infection were performed in 18 sows at 40 days of age with L.
interrogans serogroup Icterohaemorrhagiae serovar Conpenhageni strain Fiocruz L1-130, and serogroup Pomona serovar Kennewicki strain Pomona From divided in
groups: GI (control), GII (inoculated with serogroup Pomona) and GIII (inoculated
with serogroup Icterohaemorrhagiae). Clinical course of the disease, results of
serological tests and detection of bacteria by polymerase chain reaction (PCR) and
bacterial isolation during the experiment, were compared between groups.
Additionally, the main histological changes observed in brain, liver, lymph nodes,
lung and kidney of 12 animals experimentally infected and seropositive for
leptospirosis were described. In lesions suggestive of the disease the technique of
silver impregnation of Warthin-Starry, was used as direct detection method. During
the experimental period, all samples of the GI (control) were negative in the
microscopic agglutination test, with the GII positivity exclusive to serogroup Pomona
in six animals from 6 dpi, and in GIII, only to serogroup Icterohaemorrhagiae in five
animals from 3 dpi. At necropsy the only change was pale kidney in 50% of GI,
83.33% of GII and 83.33% of GIII (p> 0.05). Only in seropositive pigs was found
lesions suggestive of leptospirosis as focal microgliosis in the brain (8.33%),
lymphoid hyperplasia in lymph nodes of the mediastinum a nd mesentery (41.66%),
mononuclear interstitial pneumonia (58.33 %), multifocal mononuclear hepatitis,
further acute tubular nephrosis (100%) and interstitial nephritis (91.66%). There was
a strong association between serological condition and lesions of mononuclear
hepatitis (p = 0.0128), mononuclear interstitial nephritis (p = 0.0004), tubular
nephrosis (p <0.0001) and mononuclear interstitial pneumonia (p = 0, 0377).
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Citação
SANTOS, Adriana da Silva. Infecção experimental em suínos por Leptospira interrogans sorogrupos icterohaemorrhagiae e pomona. 2014. 87 f. Tese (Doutorado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2014.