Estudo genético e epigenético no prognóstico do câncer cervical por meio da verificação de HPV de baixo e alto risco e da metilação e não metilação dos genes RARβ, TIMP3, CDH1 E MGMT
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Data
2014-04-08
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Universidade Federal de Goiás
Resumo
The human papillomavirus (HPV) is a major etiologic factor in the development of
cervical cancer, DNA virus primarily infects the epithelium and may induce benign and
malignant lesions of the mucous membranes and skin. Carcinogenesis is a multistep
process that involves both changes genetic and epigenetic. The two changes epigenetic
most studied are DNA methylation and histone acetylation. DNA methylation may be
related development to cancer, and their presence or absence can affect the prognosis.
The objective of this study was to evaluate the prognosis of patients with cervical cancer
in stages I and II through the verification of HPV high and low risk, and the presence
and absence of genes methylated and unmethylated RARβ, TIMP3, CDH1 and MGMT.
We analyzed 129 records and samples of paraffin embedded biopsies of patients with
cervical cancer in stages I and II. Detection of HPV - DNA was performed by PCR for
HPV DNA of low and high oncogenic risk and MSP-PCR to detect the genes
methylated or not, RARβ, TIMP3, CDH1 and MGMT. The calculation of survival used
the Kaplan-Meier method and the log-hank test to compare means of survival between
the prognostic factors for cervical cancer. The overall survival at 60 months of patients
with the presence of RARβ, TIMP3, CDH1 and MGMT methylated or not were: 100%,
90.0%, 85.7%, 92.5%, respectively, and survival free of disease were 100%, 85.7%,
100%, 100%, respectively. The presence and absence of genes did not affect the overall
and disease-free survival. The prevalence of HPV of low risk patients was 45%
(58/129) and the high-risk HPV was 76% (98/129). Regarding the analysis to evaluate
the existence of statistical relationships between the presence and absence of genes
methylated or not, RARβ, TIMP3, CDH1 and MGMT with variables clinical and HPV,
it was found that there were no significant differences in the presence and the absence
of genes the methylated or not in relation to the distribution of variables. The study
demonstrated that the absence and presence of genes methylated or not, RARβ, TIMP3,
CDH1 and MGMT, did not influence the prognosis of patients suffering from cervical
cancer in stages I and II.
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D'ALESSANDRO, Aline Almeida Barbaresco. Estudo genético e epigenético no prognóstico do câncer cervical por meio da verificação de HPV de baixo e alto risco e da metilação e não metilação dos genes RARβ, TIMP3, CDH1 E MGMT. 2014. 127 f. Tese (Doutorado em Medicina Tropical e Saúde Publica) - Universidade Federal de Goiás, Goiânia, 2014.