Interações de terpenos com membranas de eritrócito, fibroblasto, estrato córneo e membrana modelo e interações de uma nanopartícula de ouro com membranas modelo
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2014-04-25
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Universidade Federal de Goiás
Resumo
The interactions of terpenes with membranes of erythrocyte, fibroblasts, stratum corneum and the model membranes of 1,2-dipalmitoylsn -glycero-3-phosphocholine were investigated by using the the electron paramagnetic resonance and fluorescence spectroscopic of lipophilic
probes. It has been shown that when added at high concentrations to systems having a high
lipid/solvent ratio, terpenes such as 1,8-cineol, α-terpineol, (+)-limonene and nerolidol are able
to self-stabilize in molecular aggregates which can extract the bilayers lipids. Studies on the
hemolytic and cytotoxic potential of various terpenes showed that cell damage caused by these
molecules are concentration dependent and that among the studied terpenes, nerolidol and
α-terpineol are the most hemolytic and cytotoxic, while (+)-limonene and 1,8-cineole are the
least hemolytic and cytotoxic. However, the low correlation between these two tests indicates
that the processes involved in each case are not completely dependent. It was also shown that
once embedded in the membrane, terpenes increase the fluidity of lipid bilayers and decrease
the temperature of the main phase transition. Differences between increased fluidity promoted
by sesquiterpene nerolidol and all monoterpenes studied were observed. Meanwhile, in a comparison of the effect of the monoterpenes studied, no significant differences in their ability to
increase membrane fluidity were detected. Furthermore, it was demonstrated by using confocal
and atomic force microscopy and fluorescence spectroscopy that the 1,2-distearoylsn -glycero-3-(Aurora nanoparticles) is better incorporated in lipid membranes under fluid phase and that
the addition of 0.1% of these conjugated nanoparticles do not produces large variations in
membrane fluidity and no causes substantial morphological changes of lipid bilayers.
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MENDANHA NETO, Sebastião Antônio. Interações de terpenos com membranas de eritrócito, fibroblasto, estrato córneo e membrana modelo e interações de uma nanopartícula de ouro com membranas modelo. 2014. 242 f. Tese (Doutorado em Física) - Universidade Federal de Goiás, Goiânia, 2014.