Desenvolvimento de dispositivos microfluídicos de papel com superfície quimicamente modificada para ensaios clínicos utilizando detecção colorimétrica
Data
2014-08-14
Autores
Título da Revista
ISSN da Revista
Título de Volume
Editor
Universidade Federal de Goiás
Resumo
This report describes the development of microfluidic paper-based analytical
devices (μPADs) with chemically modified surface for clinical assays with
colorimetric detection. The μPADs were fabricated by a stamping-based
method with a heated metal stamp for obtain hydrophobic barriers of paraffin
in paper. Before of the stamp step, the paper was oxidized to promote the
conversion of hydroxyl groups in aldehyde groups for further chemical
activation for the immobilization of enzymes. The μPADs were used for
complexometric assays of nitrite and bovine serum albumin (BSA) and
enzymatic assays of glucose and uric acid (UA). The chemical modification
did provide better color uniformity inside of the detection zones of the
enzymatic bioassays. After the chemical modification, the relative standard
deviation (RSD) values for glucose and UA assays decreased from 40 to
10% and from 20 to 8%, respectively. Clinical assays for glucose, UA, nitrite
and BSA were performed in levels which included the clinical range for each
bioassay. We performed quantitative analysis of all analytes in artificial urine
sample with error values ranged from 2,5 to 4,0%. The robustness tests
proved the stability of the chemical modification process and the thermal
stability of the μPADs when stored at different temperatures, showing the
potential of the devices as trade platforms for clinical analysis. Advantages
such as low cost per assay ($ 0.01), portability and easiness of fabrication
enable for the use of μPADs in clinical diagnostics in places with limited
resources and at the point-of-care, which is the place where the patient
requires the analysis.
Descrição
Palavras-chave
Citação
GARCIA, Paulo de Tarso. Desenvolvimento de dispositivos microfluídicos de papel com superfície quimicamente modificada para ensaios clínicos utilizando detecção colorimétrica. 2014. 100 f. Dissertação (Mestrado em Química)–Universidade Federal de Goiás, Goiânia, 2014.