Transferibilidade e variabilidade genética de marcadores microssatélites gênicos em Egenia klotzschiana Berg (Myrtaceae)
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2014-08-11
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Universidade Federal de Goiás
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Eugenia klotzschiana Berg is a species of Myrtaceae with restricted distribution in the Cerrado. Its fruits present nutritional potential and can be used in extractive way, as raw material for production of jams, jellies and juices. In this context, it is necessary to evaluate population-genetic aspects in order to provide information to assist in targeting strategies for management and conservation. To assess the genetic variability in a population context microsatellite markers have been used in recent years. The sequences flanking the microsatellite regions of the genome are highly conserved between related species, enabling the portability of these markers, reducing thereby the costs of developing the same for each species under study. In this context, the aim of this study was to test the potential for transferability to the genome of E. klotzschiana of genic microsatellite markers developed for Eucalyptus, as well as to characterize the genetic variability in their populations. For this purpose, DNA was extracted from leaf tissue of E. klotzschiana individuals and used to test the cross 120 pairs of amplification primers. Samples from seven locations were used to characterize the population genetic variability. The amplification products were analyzed on agarose and polyacrylamide capillary electrophoresis gel in different stages. Of total primers tested, 67 (55.83%) did
not amplify, 42 (35%) had many nonspecific amplification products and 11 (9.2%) were
successfully transferred. Of the 11 transferred eight showed polymorphism. For the
eight polymorphic markers, the probability of identity was high (2.02 x10-3) and the
power of paternity exclusion was moderated (0.74). In this population the average value
of expected heterozygosity (He) was 0.28 and observed heterozygosity (Ho) was 0.44,
as in subpopulations of He mean values ranged from 0.18 to 0.27 and the mean values
Ho ranged from 0.36 to 0.53, respectively. No significant for the fixation index (f) were
observed in the populations values indicating allele frequencies below the expected
Hardy-Weinberg proportions.The extent of genetic divergence among subpopulations
showed relatively high genetic differentiation, with a value of θ equal to 0.20, and peerto-
peer values ranging between 0.00 and 0.34. The strategy of transferability of
microsatellite markers was efficient to generate a panel of polymorphic microsatellite
markers and learn a little of the genetic variability of the species. It was possible to
verify that there is a low genetic variability within subpopulations for biomarkers.
However, this work is a starting point for future studies to better understand the
reproductive biology of the species Eugenia klotzschiana.
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SIQUEIRA, M. N. Transferibilidade e variabilidade genética de marcadores microssatélites gênicos em Egenia klotzschiana Berg (Myrtaceae). 2014. 81 f. Dissertação (Mestrado em Genética e Biologia Molecular)–Universidade Federal de Goiás, Goiânia, 2014.