Avaliação da atividade antioxidante, hipotensora e vasodilatadora da Jabuticaba, Myrciaria cauliflora Berg.
Carregando...
Data
2014-09-30
Título da Revista
ISSN da Revista
Título de Volume
Editor
Universidade Federal de Goiás
Resumo
The research and the use of medicinal plants as therapeutic agents has been increasing in recent years and the jabuticaba (Myrciaria cauliflora) has occupied a favorable place in this segment by presenting biological properties due to their high antioxidant activity due to the presence of phenolic compounds. The aim of this study was to investigate the phytochemical characterization of EHAJ, evaluate the antioxidant potential of HAEJ and evaluate the influence of HAEJ on hemodynamic parameters in rat (in vivo) and the mechanisms involved in variation in vascular tone caused by HAEJ in aortas isolated of rat (in vitro). The quantification of the phenolic compounds in HAEJ was performed by a method that is based on the complexation of phenolic compounds present in the solution of ferric chloride (FeCl3) as measured by a spectrophotometer and ellagic acid quantitation was performed by analysis high performance liquid chromatography (HPLC). The phytochemical characterization of HAEJ was evaluated considering only one methodologie (mass spectrometry - ESI-MS) and antioxidant activity of HAEJ was evaluated considering two different methodologies (DPPH and differential pulse voltammetry - DPV). To achieve the experimental protocols in vivo, were inserted with the animals anesthetized, catheters in the right femoral vein and artery, and flow probes around the aorta for infusion of EHAJ, check blood pressure, heart rate and aortic blood flow, respectively. Besides the pressure was checked the vasorelaxant effect of HEAJ in preparations of isolated rat aorta pre-contracted with vasopressor agents. The concentration of nitric oxide produced by the endothelial cells in the presence of HEAJ was also evaluated by flow cytometry. Intravenous infusion of HEAJ (0.12 to 0.96 mg / kg) produced dose-dependent increase in aortic blood flow hypotension. In isolated arteries pre-contracted with contractile agonist, HEAJ induced concentration-dependent relaxation which was potentiated in preparations with intact endothelium (E+) (120μg/ml) as compared with preparations devoid of endothelium (E-) (1.92 mg / mL). And preparations pretreated with L-NAME, ODQ and MDL-12,330A reduced the relaxation caused by HEAJ, whereas pretreatment with diclofenado, atropine and propranolol did not alter the activity of HEAJ. In E- preparations pretreated with TEA, 4-AP, glibenclamide and ODQ, relaxation caused by HEAJ was reduced, while the clotrimazole pretreatment with CPA and does not alter the activity of HEAJ. Furthermore, HEAJ was able to decrease phenylephrine and KCl induced contractions. These findings suggest that the hypotension associated with HEAJ induces vascular relaxation; also suggests the involvement of such pathways cGMP / sGC and cAMP / AC and finally the involvement of potassium channels and calcium channels, this relaxation.
Descrição
Palavras-chave
Citação
ANDRADE, D. M. L. Avaliação da atividade antioxidante, hipotensora e vasodilatadora da Jabuticaba, Myrciaria cauliflora Berg. 2014. 100 f. Dissertação (Mestrado em Ciências Farmacêuticas) - Universidade Federal de Goiás, Goiânia, 2014.