Expressão de receptores de EGF, inibidores e reguladores do ciclo celular em lesões proliferativas da próstata canina
Carregando...
Data
2014-11-14
Título da Revista
ISSN da Revista
Título de Volume
Editor
Universidade Federal de Goiás
Resumo
Somedysplastic lesionsof prostate, such as proliferativeinflammatoryatrophy (PIA) and
prostatic intraepithelial neoplastic(PIN) are proliferative,consideredpremalignant andcan
progress toprostatic carcinoma (PC). Others, such asbenign prostatichyperplasia(BPH) are
proliferative, butare not related tomalignancy.The humanPC is the one ofcancers that more
kill,and dog`sthe onlyanimal species thatpresentsadiseasewith similarcharacteristics, thishas
been usedas a modelfor studyof prostatecancer. Thus, in order tobetter understandthe
molecular mechanisms involvedin repairand cell cycleevolution ofproliferative
lesionsincanine prostate, this study aimed to evaluatethe gene expression ofErbB1, ErbB2,
CDKN1A, CDKN1BandTP53andimmunostaining ofEGFR(Her1), Her2(c-erbB-2), p21,
p27and p53 inprostateswithPIAandPC. Also was evaluatedtheimmunostaining ofp21, p27,
p53, c-myc andcyclinD1inprostateswith BPH, PIA, PINandPC. For this purpose,wereobtained
from atissue microarray (TMA)block, 106samplesofcanineprostate tissue, which 15 normal,
16with BPH, 30 withPIA, 20 with PIN, and 25with CP. The RT-PCR andRT-qPCR
techniques wereused to evaluategene expression, as well as immunohistochemistrywas used
to determinetheimmunophenotypeofprostatic lesions. As forEGFRand Her2has beenobserved
that thesereceptorsactin canine lesions withPIA and with PCan importantaction ofHer2,
suggesting that theyare involvedin carcinogenesisandtumor development in canineprostate.
Regarding to cellcycleinhibitor concludethat whenoverexpressedin canine
prostateswithPIAandPC, p21andp27control cellproliferation, acting as a protective factorin
the evolution of PIA to PC, and in the PC development, even in the presence of altereted p53.
In assessing ofimmunostaining ofcell cycle regulators(p21, p27, p53, cyclinD1andc-myc) in
canineprostateswithproliferative lesionswas observedthatcarcinomas probably have
lowgrowth potentialwhenoverexpressed of p21andp27, reduced expression of cyclinD1and
unalteredexpression ofc-myc, even in the presence ofmutant p53type.Further,considering
thesimilarimmunophenotypein canine glands with PIA, PINandPC considered the
regulatorsofcell cycle progression, it is reinforce the pre-malignant potential of PIA andPINin
the canineprostate.
Descrição
Palavras-chave
Citação
FALEIRO, M. B. R. Expressão de receptores de EGF, inibidores e reguladores do ciclo celular em lesões proliferativas da próstata canina. 2014. 103 f. Tese (Doutorado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2014.